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Interaction Of PAAC From Dune Reed(Phragmites Communis Trin) With Chloroplasts Of Swamp Reed And Effects On Membrane Fluidity And Oxidation Injury

Posted on:2011-09-25Degree:MasterType:Thesis
Country:ChinaCandidate:Y X ZhangFull Text:PDF
GTID:2120360305465906Subject:Botany
Abstract/Summary:PDF Full Text Request
The experiment studies on the adsorption characteristics of a novel small compound specified PAAC in dune reed and chloroplasts of swamp reed as well as the effects on membrane fluidity and oxidation injury through adsorption experiment, membrane fluidity and oxidation injury method with swamp reed (Phragmites communis Trin)material. A fluorescence spectrophotometry method was performed to research the change of membrane fluidity of chloroplasts treated by PAAC. We Studied the change of oxidation injury of Chloroplast membrane lipids via various treat time(0,1,2,3h) and temperature(25℃,35℃), the results were summarized as follows:1. Our data suggest that the absorption of PAAC by chloroplasts suits alternate distribution, the partition coefficient is 0.7, the quantity of adsorption has a upward trend with the pH changes high, when pH=7.6, the quantity of adsorption is the highest(0.86μg/μg),the ionic strength change have no significant influences the quantity of adsorption.2. The results of the study indicated that membrane fluidity of chloroplasts have been enhanced after treated by PAAC, furthermore, it is a negative correlation relationship.3. Resulted suggest PAAC can decrease the membrane lipid oxidation injury when treated by strong light intensity and high temperature, and this protection function is in parallel with the concentration of PAAC. With PAAC concentration increasing from 0 to 25μol/L, the MDA quantity declined rapidly, This protection effect was also confirmed by removal hydroxyl radical(-OH) experiment.
Keywords/Search Tags:PAAC, chloroplasts, interaction, adsorption, membrane fluidity, oxidation injury
PDF Full Text Request
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