| Resonance Rayleigh scattering (RRS) is a new analytical technology developed in1990s. For its excellent sensitivity, simple operation, cheap instrument and rapidly analytical speed, this method has received more and more attentions and interest of scientific and technological workers.Resonance Rayleigh scattering (RRS) is based on the strong RRS signal caused by the effects such as the aggregation of the chromophore on biomacromolecules and ion-associated reactions, which are formed by hydrophobic force, charge transfer force and electrostatic between small molecules. This method has been used to study protein and nucleic acid ,and also used to determine some trace amount of inorganic metallic ion and organics. The method of RRS has a good applications and perspe- ctive on environmental science, life science and nano-meter materials because of its high sensitivity.This thesis is divided into two parts, the first is the literature review, introduces physiological functions of the vitamins B1, B2, B12 and their common analytical methods, and also introduces the development and application of resonance Rayleigh scattering technology. The second part reported the method of determination for vitamin B1, B2 and B12 with resonance Rayleigh scattering, the main contents are as follows:1. The resonance scattering spectra of Orange G and vitamin B1 system has been studied in the presence of pH=3.0 HCl-NaAc. The spectral characteristics and the effect factors have been investigated. The RRS intensity is directly proportional to the concentration of vitamin B1 in the range of 0～11.2μg·mL-1 with a correlation coefficient of 0.9982. The detection limit is 6.9×10-4μg·mL-1, the relative standard deviation is 1.60% (n=10), the recovery is between 92.6～106.9%. Thus, a method of RRS for determination vitamin B1 is developed.2. The resonance scattering spectra of BCG and vitamin B1 system has been studied in the presence of pH=2.5 HCl-NaAc. The spectral characteristics and the effect factors have been investigated. The RRS intensity is directly proportional to the concentration of vitamin B1 in the range of 0～9.4μg·mL-1 with a correlation coefficient of 0.9975. The detection limit is 2.96×10-4μg·mL-1, the relative standard deviation is 0.92% (n=8), the recovery is between 92.7～107.3%. Thus, a method of RRS for determination vitamin B1 is developed. 3. The resonance scattering spectra of vitamin B2 and SDS system has been studied in the presence of pH=6.0 BR. The spectral characteristics and the effect factors have been investigated. The RRS intensity is directly proportional to the concentration of vitamin B2 in the range of 0～14.2μg·mL-1 with a correlation coefficient of 0.9990. The detection limit is 6.1×10-4μg·mL-1, the relative standard deviation is 1.09% (n=6), the recovery is between 92.5～105.5%. Thus, a method of RRS for determination vitamin B2 is developed.4. The resonance scattering spectra of cobalt (Ⅱ) with thiocyanate and Neutral Red have been studied in the presence of PVA-124.The spectral characteristics and the effect factors have been investigated. The RRS intensity is directly proportional to the concentration of cobalt in the range of 0～0.48μg·mL-1 with a correlation coefficient of 0.9989. The detection limit is 4.4×10-5μg·mL-1. Thus, a method of RRS for determination of trace amount of cobalt is developed.
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