The DNA-binding And Anticancer Activity Studies Of Heterocyclic Ruthenium(Ⅱ) Polypyridine Complexes

In recent years, studies of interaction of ruthenium(II) polypyridyl complexes with DNA have aroused comprehensive interest because of their rich photochemical, photophysical and electrochemical properties. These studies on the binding mechanisms of these complexes with calf thymus DNA are so important to screen out the diagnosis reagent and chemical therapy medicine of some diseases related to gene and virus in clinic medicine.This thesis consists of four chapters.In chapter one, The theoretical foundation, the current situation, the research methods of interaction between ruthehium(II) polypyridine complexes and DNA were introduced. And we summarized the research significance of this thesis.In chapter two, a new intercalative ligand nftp which includes–NO2 group and its Ru(II) polypyridyl complexes [Ru(phen)2(nftp)]2+ and [Ru(bpy)2(nftp)]2+ have been synthesized, characterized. The DNA binding properties of these complexes have been studied with viscosity measurements, spectra, CD spectra and photoactivated cleavage of DNA. The results indicate that both complexes bind to DNA via an intercalative mode and two complexes could interact with DNA enantioselectively. The results suggest that the ancillary and–NO2 group have significant effect on the complexes.In chapter three, a new intercalative ligand ttbd and its Ru(II) polypyridyl complexes [Ru(phen)2(ttbd)]2+(1),[Ru(bpy)2(ttbd)]2+(2) and [Ru(phen)2(ttbd)]PtCl24+(3) have been synthesized, characterized. The DNA binding properties of these complexes have been studied with viscosity measurements, spectra and DFT. What's more,we use ion titrating,pH titrating and anti-cancer test in vitro by MTT method to study these complexes'properties. The results indicate that all complexes can bind to DNA via an intercalative mode; complex(1) can used to be the pH sense, ions and water's recognization; Preliminary results indicate that these complexes show high activity against HepG2 and HL-60, which can help pick out the efficient anticaner medicines.In chapter four, a new intercalative brige ligand btbtp and its Di- Ru(II) polypyridyl complexes[Ru(2,9-dmp)2(btbtp)Ru(phen)2]4+(1),[Ru(2,9-dmp)2(btbtp)Ru(2,9-dmp)2]4+(2) and [Ru(2,9-dmp)2(btbtp)Ru(dmb)2]4+(3), `have been synthesized, characterized. The DNA binding properties of these complexes have been studied with viscosity measurements and spectra. What's more, we use anti-cancer test in vitro by MTT method to study these complexes'properties. The results indicate that all complexes could bind to DNA via an intercalative mode; Preliminary results indicate that these complexes show high activity against HepG2 and HL-60, which can help pick out the efficient anticaner medicines.