Seperation Of Vitamin E And Phytosterol From Cottonseed Oil Deodorizer Distillate

Cottonseed Oil Deodorizer Distillate(CODD) is the byproduct of cottonseed oil refining process, mainly containing free fatty acid, natural vitamin E, phytosterols and glyceride et al. Natural vitamin E and phytosterols due to their physiological activity play major roles in several areas, namely in pharmaceuticals, food , cosmetics and feed. The purpose of the thesis is to study technics of separating vitamin E and phytosterols from CODD. The operation condition of specific adsorption and crystallization for separation vitamin E and phytosterols respectively have been studied.According to the National Standard, the physicochemical properties of CODD have been analyzed as follows: water and volatile matter 0.920%, acid value 71.1 mgKOH/g, saponificaton value 133 mgKOH/g, glyceride content 35.0%, vitamin E content 5.97%, phytosterols content 12.3%.In the same condition, macroporous resin D1 has better performance of specific static adsorption.Through dynamic adsorption in a fixed bed, adsorption agents and the flow rate of ethanol have been compared. The results showed that CO2-ethanol as desorption agents is better than 5%(v%)acetic acid-ethanol, and the suitable flow rate is 0.5BV/h. In the optimal condition, the purity and yield of vitamin E are 76.6% and 91.6%, respectively. After readsorption, the purity and yield of vitamin E are 78.7% and 99.4%, respectively.Using the adsorption by-product as material, the optimal conditions for crystallization are: the solvent of ethyl acetate; the proportion of crystallization material to the solvent= 1:5(weight to volum); the temperature -5℃; the time for cooling 96 h. The experiment results of recrystallizingphytosterol in ethyl acetate showed that the purity and the yield were 77.3% and 52.1%,respectively.