A Study On Development Of Buffalo Immune Milk

4 kinds of human medical vaccines (to hepatitis A, cholera, dysentery and typhoid fever, respectively) were used to intensively immunize the Holland cows and milk buffalo. The phases of relevant anti-bodies in the milk appeared after immunization were observed and some of the deference between the immune milk from Holland cows or buffalo was compared. Technological process of drawing the immune substances from the immune milk was investigated, and the bacteriostasis effects of the immune substances from the immune milk were defined. Results showed (1) Antibodies to hepatitis A could be detected from blood of Holland cows as well as buffalo one week after immunization and it could be detected from milks produced by the two kind of animal after immunization. The activity peaking of antibodies in the buffalo milk could he seen in the 18th week (9 weeks after immunization), one week later than that in cow milk. The peaking activity values of both milks could maintain for about one week. Antibodies to vibrio cholerae , to bacilli of typhoid fever or dysentery, appeared one or two weeks after immunization, with relatively low activity value. Among them, the activity of antibodies to vibrio choierae and to bacillus of typhoid fever could last for 5 weeks, while that of antibodies to bacillus of dysentery could last for week only. (2) Ammonium sulfate graded segregation could be used to draw antibodies from immune milk with satisfy effect. Saturation of ammonium sulfate should be changed from 35% to 25%, then to 35% again, in the rude segregation. The optimal saturation of ammonium sulfate was 33% in fine segregation. DE-52 fiber column chromatography could be used to further purifying of the immune products. (3) Bacteriostasis effects to vibrio cholerae and to typhoid fever bacillus could be seen when the density of the rude product of immuno-giobulin (Ig) reached 1.0mg/mi in the culture.