Study On Energy Transfer's Fluorescence Analysis Between Basic Dyes

This paper consists of five chapters. In the first paper, Application of fluorescence analysis based on resonance energy transfer (FRET)is reviewed with 56 references. The review includes Fster theory of fluorescence resonance energy transfer, analysis techniques and application. In the second chapter the fluorescence energy transfer between some basic dyes, such as acridine orange (AO), acridine yellow (AY), rhodamine 6G(Rh6G), rhodamine B (RhB), ethyl ester of rhodamine B (ERhB), buthyrhodamine B (BRhB) and safranine T (ST), was investigated. The effect of various surfactants on fluorescence energy transfer of basic dyes was studied, too. The conditions effecting energy transfer efficiency were discussed. Practical application of fluorescence energy transfer to the determination of inorganic ions was introduced in the third chapter. In the first section, the determination of trace phosphorus by using energy transfer fluorescence quenching from acridine orange to rhodamine 60 was studied. In the second section, study on the energy transfer florescence qtienching among combination of acridine yellow-PAR determination of trace iron. In the fourth chapter, determination of trace Vanadium(v) by a fluorescence quenching method with rhodamine B-7-iodo-8-hydroxyquinoline-5-sulfonic acid surfactant system was studied. In the fifth chapter, spectrofluorometric determination of trace germanium with phenylfluorone in flyash was investigated.