| Cyanine dyes bear the merits of large molar absorbability and high fluorescence quantum yield. Meanwhile, the spectra of cyanine can be extended from visible region to near infrared (near-IR) by tuning the length of the conjugate chain. And a better sensitivity and selectivity would be obtained if the measurement is performed in near-IR region, a region of little interference from background. Furthermore, cyanine is sensitive to environment and its spectra may be varied with the change of microenvironment, which makes it very suitable for many analytical missions. In fact, cyanine has been already become one of the most important probes in fluorescence analysis field. In this dissertation, I would continue to develop the application of cyanine in the fields of environmental and biological assays on the basis of previous work of predecessor. The dissertation consists of seven chapters.In chapter 1, classifications, synthesis methods, various qualities and application of cyanine were firstly summarized. Emphasis was focused on aggregation qualities of cyanines and their recent research progress in the fields of surfactant system, biological system, and fluorescence sensor. Then, the research proposal for this dissertation is presented.In chapter 2, the apparatus, reagents and synthesis approaches of some cyanine and phthalocyanine compounds used in this work were described.In chapter 3, the spectral behavior of cyanine in the presence of surfactants was studied. Firstly, the fluorescence changes of cyanine I and II with the concentration of surfactants around CMC were investigated, respectively. The results showed that the fluorescence of the hydrophobic cyanine II was completely quenched in water and aqueous solution containing a low concentration ( |
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