A Study On Selection Of Heavy Metal Resistant Strains And Its Biological Function

After isolation of monoploids, mutation with UV and NTG, We abtained two strains from Saccharomyces cerevisiae Yl and Y3: SYl-4-14-5(the resistance level of CuSO₄ is 8mmol/L) and SY3-2-14-2 (the resistance level of CdCl₂ is Immol/L) .The genetic stability of these strains were over 90%.By SephaxG-50 and DEAE-52 then SephaxG-25 for desalination , metallothionein was isolated and purified.We analyzed and compared the biological function of reducing toxicity to heavy metal, resisting the irradiation of UV and ^Co and clearing -OH between these strains. It seemed that a positive correlation between the biological activity and resistance of yeast cells to Cu2+ or Cd2+ was observed. The mutants showed higher biological activity.