Studies On The Preservation Of Low Temperature-heated Sausage

On foundation of flora analysis of cooked and smoked sausage, five separated strains are tested by using five antiseptics. The effect of five antiseptics and their mechanisms of bacteriostasis are studied and optimum parameters of the antiseptics applying in low temperature-heated sausage are gained.This research comprises three parts.In the first part, the main bacteria groups which can cause sausage spoilage were analysed and the pure culture of spoilage bacteria was gained.The second part acquire optimum parameters by bacteriostasis experiment of five antiseptics.In the third part, two regression equations were established through saturation D-optimum design and the optimum parameters were obtained and tested.The main testing results are as follows:1.ln the low temperature-heated sausage, lactobacillus were dominant bacteria, sharing 89.7% of the total amount.While lactococcus, enterococcus and bacillus were small, only sharing 9.8% of the total amounts.thermosphacta, enterobacteria and yeast were not detected.AII the bacteria separated from low temperature-heated sausages can grow well in the pH range of sausages.2.The bacteriostasis of sodium lactate, sodium propionate, potassium sorbate, nisin and chitosan oligomer was studied in single factor test.lt shows that each antiseptics have bacteriostasis on the bacteria in choosed concentration, but it is not consistent in different strains. 2% sodium lactate are the best dosage used , and so do 0.225% potassium sorbate, 0.03% nisin and 0.06% chitosan oligomer. Sodium propionate has a little range of bacteriostasis, and limits its use in sausages. S.Considered the outcome of single factor test, two regression equations were established by applying saturation D-optimum design in which X1 is equivalent to code value of sodium lactate, X2 is equivalent to code value of nisin, X3 is equivalent to code value of chitosan oligomer, Y is equivalent to TVBN and Z is equivalent toLogarithms value of CPU.Y=8.5584-1.0453X1-0.5604X2+0.1118X3+0.3695X1X2+0.4507X1X3+0.5938X2X3+0.46 75X12+0.5183X22-0.8308X32 2=8.5090-0.1667X1-0.1045X2-0.0629X3-0.0041X1X2+0.0363X1X3+0.0112X2X3+0.107Through F-test, the result is F>F0.01 , which shows that two equations are resonable.By further analysis, we draw the conclusion that Y can reach the minimum value when the code values of X1 , X2, X3 are 1 , 0.7570, -1 and Z can reach the minimum value when the code values of X1 , X2, X3 are 0.7244 , 0.6036 , 0.3779. 4.The experiment showed that TVBN and Logarithms value of CFU can decrease evidently by using the optimum parameters of three antiseptics.