| Through studying on the correlation of the inhibition zone of agar column against Stremptomyces scab with the production of antibiotics, the initial screening method of rejuvenated strains of Men-myco-93-63 was established. Based on this, comparisons were made among five rejuvenating media and oat media. Asp-oat media was selected. Through three-grade selecting , two high-yield strains y-13, A-25 and a strain A-l 9 with different cultivated character was obtained.Culture filtrate from the No.4 medium inoculated with Men-myco-93-63 was suppressive to the growth of the pathogen causing cotton verticillum wilt. The two main active substances were extracted and purified from it. The crude material was extracted with macroreticular absorbent HZ-803 and further purified by column chromatography and reverse-phase high-performance liquid chromatography (HPLC). The figure of HPLC spectrum of the material purified show that men-myco-93-63 can produce 13 active components at least. Among them, two peaks are pure. The two components scanned with UV detector indicated that the maximum absorbing peaks of peak 20 and component 21 are 220nm and 280nm. respectively. |
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