Competition Binding Equilibrium Study Between Mn(Ⅱ), Zn(Ⅱ) And HSA Or BSA Analysis On Fluorescence And Resonance Scattering Spectra Of Silver Nanoparticles And HSA Or BSA

PartiThe cometition binding equilibrium of Mn (â…¡),Zn (â…¡) inos with HSA or BSAis studied by the method of equilibrium dialysis at physiological pH(7.43).The states of competition binding between Mn (â…¡),Zn (â…¡) and HSA or BSA were comprehensively analysised by three different addition sequence,the similarity and difference were compared with there single metal systems . The successive stability constants which are obtained by non-linear least-squares methord fitting Bjerrum formula. In three- system ,the results from Scatchard plots indicate that there exists two strong binding site of Mn (â…¡) and one strong binding site of Zn (â…¡),there are same results compareing with two -system ,but some more weak binding sites are different from the different addition sequence ,show that there are not common strong binding site of Mn (â…¡),Zn (â…¡) in HSA or BSA .Strong binding sites have electiveness and unfalteringness ,weak binding sites exists irreversibility.Competion results show that there exists clear positive cooperative effect between Mn (â…¡) and Zn (â…¡) binding with HSA or BSA,The analysis of Hill plots and free energy coupling support this conclusions.Part 2The interaction of serum albumin with Silver Nanoparticles has been studied by the fluorescence spectra and the resonance scattering spectra ,the enhancement of fluorescence occurs when concentration ratio of silver nonoparticles and serum albumin is low ( yellow[Ag] [BSA]<1,green[Agj [BSA]<0.1 ),the quenching occurs when concentration ratio is high. More big Particle diameter is ,more obvious the quenching of fiuorence is,the resonance scattering is more and more stronger with proportionate increasing. Adsorption between silver nonoparticles and serum albumin shows that serum albumin binds to silver nonoparticles mainly by electrostatic force.The mechanism of the fluorescence enhancement and quenching are analysised ,the quenching constants are calculated,,...