Effects Of Uniconazole, Brassinolide And Plant Extracts Applied In Rape Seed Coating Agent

The effects of seed film coating with suitable concentration of uniconazole and brassinolide on growth and physiological and biochemical changes of seedling in rape, cv. Zheyou758. Shuang72 and Huaza4, were investigated after selecting suitable concentration of uniconazole and brassinolide in seed film coating agent. Effects of different plant extracts on seed bom fungi of rape and on growth and physiological characteristics of seedling after seed film coating with natural matter coating agent in rape were also studied. Field experiments researching effects of coating agent. Coat I. Coat n and Coat III, on growth and yield after seed film coating were conducted. Main results obtained are as follows:Rape coating agents with different concentration uniconazole were used for film coating three rape variety seeds. Suitable concentration of uniconazole in coating agents (Coat I) was selected after germination, chlorophyll content, shoot height and dry weight were estimated. Results in laboratory showed that seed film coating with suitable concentration uniconazole did not improve energy of germination, germination percentage and simple vigor index, slightly reduce seed germination rate. However, seed film coating with suitable concentration uniconazole significantly reduced shoot height and significantly increased root length, root volume, root dry weight, cotyledon dry weight and dry weight ratio of root to shoot at the same time. Seed film coating with suitable concentration uniconazole significantly reduce Zheyou 758 and Shuang 72 lipase activity and significantly increased activity of POD, CAT in Shuang 72 and Huaza 4 seedling and PPO in three variety rape seedling. There were no significant difference between Coat I and controls in SOD activity, however significant increasing in root activity and soluble sugar content of rape seedling after seed film coated with suitable concentration uniconazole. There was positive effect of interaction on increasing activity of POD and CAT in Shuang 72 and Huaza 4 and PPO activity, root vigor and soluble sugar content in rape seedling of three varieties between Uniconazole and base coating agent (Coat 0).Rape coating agents with different concentration brassinolide were used for seed film coating in three rape varieties. Suitable concentration of brassinolide in coating agents (Coat H) was selected after germination, chlorophyll content, seedling height and dry weight were measured. The results showed that seed film coating with suitable concentration brassinolide did not improve energy of germination, germination percentage and simple vigor index, slightly reduce rape seed germination index and increase Shuang 72 mean germination time (MGT). Film coating with suitable concentration brassinolide increased cotyledon dry weight. There is no significant effect on stem dry weight, root dry weight, total dry weight and dry weight ratio of root to shoot and inhibiting root length in Zheyou 758 and Huaza 4. Film coating with suitable concentration brassinolide inhibited effect of basic seed coating agent (Coat 0) on improving root vigor in Shuang 72 and Huaza 4. Coat II significantly reduced -amylase activity Zheyou 758,however improved -amylase activity in Shuang 72 and Huaza 4 and seedling lipase activity and protease activity in three varieties. Film coating with suitable concentration brassinolide increased activity of CAT, PPO and SOD and soluble sugar content of rape seedling.Various plant extracts could significantly restrain seed bom fungi of rape. Autoclaved leek, capsicum extracts and unautoclaved garlic extracts could restrain seed bom fungi of rape completely. It suggested that these extracts could be selected to substitute pesticide and fungicide used in seed coating agent. Seed film coating with 1% chitosan solution significantly increased energy of germination, fresh root weight, the activity of lipase, protease, CAT, SOD and PPO and root vigor. There was not significant influence in germination percentage and fresh shoot weight. Seed film coating w...