Study On Optics Character And Determination Of Glutathione

Glutathione , a tripeptide with one sulfhydryl group ,is present in many physiological fluid , animal and microbial cells. It plays important biologic function in the organism ,including protein and DNA synthesis ,enzyme activity, metabolism and cell protection. And It has very wide applications in the life sciences, food, environment protection , physic field and so on. Essential to ongoing investigations of this tripeptide are assay techniques which are sensitive, specific, and easily perform with readily available materials.The optics character of GSH had been studied in this work. The new detect methods of GSH had been established. And the effect of metal ions on fluorescence intensity of GSH-OPA was studied too. The result is as follow.1 The fluorescence system of GSH-OPA has good fluorescence signal with phosphoric acid buffer (pH8.0) . And GSH could be determined using OPA with linear range of 2.8 × 10-8-9.8× 10-6mol/L. The detect limit is 2.8 × 10-8 mol/L, presenting relative standard deviation 4.3 percent.2 The effect of lanthanum ions ,zinc ion, plumbum ion, copper ion on fluorescence intensity of GSH-OPA had been studied. And the result shows that only zinc ion can enhance the fluorescence intensity of GSH-OPA. Simultaneity, the zinc ion also can improve stability . On the basis of zinc ion can improve fluorescence intensity of GSH-OPA, the determination of GSH had been established, with low detect limit.3 Two new fluorescence intensifiers of GSH had been found, 4-aminosulfonly-7-fluoro-2,l,3-benzoxidiazole and Fluram. Then using the two intersifiers,GSH could be determined with the linear range of 5.6-10-8-8.4× 10-6mol/L and 4.2 × 10-7-9.8× 10-6mol/L respectively, with determination limit 5.6 -10-8 mol/L and 4.2 ×10-7 mol/L.4 The new methods were applied to detect GSH in samples . Moreover, the methods were contrasted with HPLC, with satisfactory results. with easily operation and low determination limit.