Investigation On The Galvanic Cell Generated Electrochemiluminescence And Its Analysis Application

This thesis includes a review and a research section. The review includes the history, principle, ECL systems, recent developments of ECL and trend towards miniaturization of ECL equipment. The research section contains a brief conclusion of our research work and four research subunits. In the research section, we have investigated on line galvanic cell and micro-batteries generated electrochemiluminescence. Using on line galvanic cells, we determined isoniazid, Ca2+, aciclovir; Based on micro-batteris generated ECL, we have investigated hydroperoxide-luminol ECL system. Experiment result implied this model of ECL detection could be applied for instrument miniaturization with easy fabrication.The phenomenon of luminescence upon electrolysis could be dated back as far as 1927 for the ECL of Grignard compounds at applied potentials, ECL proved to be an important and valuable method for analytical applications with increasing interest over the past two decades, this also could be seen from the growing number of reviews in this period. When reviewing the analytical use of ECL, the features of ECL could be seen as following: firstly, owing to the absence of an excitation light source produces a low background, the optimization of material, size and position of the electrodes and the variations of the applied potential, high sensitivity and selectivity could be achieved; secondly, the in situ activation of compounds could be used to generate reactive co-reactants and allowed the detection of a wider range of analytes; thirdly, comparing with CL, the addition of reagents in separate stream could be eliminated and this also resulted the improvement of sample dilution and expensive of instrumentation. Because of the high sensitivity and selectivity, ECL was also thought to be a suitable method for the detection on microchip. Up to now, employing an external potential supplier carried all of the reference technique for ECL generation out. Considering of the size and expense of the potential supplier normally used in research work, it would limit the detection instrumentation miniaturization or even further application of ECL detection on a chip. Miniaturization of conventional analytical equipment offers many advantages such as short reaction time, safe handling, and safe storage of small quantities, lowconsumption of reactants, automation, and integration of separation and detection on the same chip.The research section includes four sections: The former three sections of which are based on the on line galvanic cell generated chemiluminescence. Candidate metals such as aluminum, zinc, chromium and cadmium were used as pure metallic anode, copper, silver, gold, platinum, and graphite for cathode of galvanic cell were investigated. At last, considering of the expense of the cell fabrication, the stability of output potential, we choose Al as anode and Ag as cathode. It supplied a stable potential output in a flow of sodium hydroxide solution. The potential variation in about 1300 mV to 560mV range could be obtained by varying the concentration of sodium hydroxide or by employing different alkaline solution in the flow. When the potential output of the described cell was 1168 mV while the sodium hydroxide concentration was 5.0+10-4 mol/L and luminol concentration was 6.0+10-6 mol/L, the presence of isoniazid could greatly enhance the electrochemiluminescence of luminol in the vicinity of platinum anode surface. Isoniazid contents in three kinds of isoniazid tablets were determined and the analysis results agreed well with those from official method. This analysis application proved to be a practical use for presented method.This on line galvanic cell was investigated and applied to the ECL determination of calcium content in milk and vegetable samples, the potential output of the described cell was 1070 mV while the flow reagent constitute was 0.18 mol/L concentration of sodium hydroxide and 2.0+10-5 mol/L concentration of calcein blue. Based on the weak ECL of calcine blue could be greatly sensitized in the...