Study Of Schizophyllan

The fermentation conditions, the extraction methods and the structure of schizophyllan were studied in this thesis. The main contents and results were as follows:Fermentation schizophyllan by Schizophyllum commune was optimized. The results of single factor and orthogonal experiment showed that the optimized medium composition contained: glucose 45g/L,yeast extract 3g/L,KH2PO4 0.5g/L,MgSO4.7H2O 0.5g/L. The optimized conditions were inoculum age 66h,inoculum size 10%,temperature 27 C,volume of medium 20% and pH 6.Maxium yield of schizophyllan and mycelium of flask were 2.70g/L and 20.75g/L respectively .But the yields of schizophyllan and mycelium were 0.84g/L and 8.81g/L respectively in batch culture. The result of water-fed experiment showed that the yields of mycelium and schizophyllan were greatly improved and them were 19.98g/L and 1.89g/L respectively.The extraction method of exo-polysoccharide from schizophyllum commune by submerged cultivation was studied. The extraction technology was improved and the best methods of removing color and Proteins were achieved.The structure of schizophyllan from schizophyllum commune by submerged cultivation was also researched. The purified schizophyllan was proved to be homogeneous with molecular weight 4 + 104u by sephadex G-200 column chromatography, PAGE and HPLC. Its monomer was determined by hydrolysis, PC, GC and its structure was analyzed by IR, enzymolysis, periodate oxidation, CP/MAS13C NMR, the results showed that schizophyllan was only composed of glucose and it consisted of a backbone chain of 1,3-B-D-glucopyranose units linked with single 1,6-B-D-glucopyranoses at about every third glucose molecule in the basic chain.