The Technical Research Of Extraction & Purification Of The Bamboo Leaves Flavonoids

Firstly, the total flavonoids content of the bamboo leaf was determined in this paper. It was concluded that the total flavonoids content of per gram dry bamboo leaf was 13.65 milligram, the result was basically consilient with the result which was reported by reference literature. In this literature, the range of 5.9-13.1 mg/g of the total flavonoids content of bamboo leaf was determined by methanol extraction , taking WYGC18 as chromatogram column, taking methanol: water: phosphoric acid(60:40:0.5)(v:v:v) as mobile phage, and taking 254nm as determining wavelength.Secondly, the optimal extracting conditions of bamboo leaf flavone(the abbreviation is BLF) were studied, and it could be determined that alcohol soaking was the best extraction method and 64% alcohol(v/v), the 1:30(w/v) of material and alcohol ratio, 6 hours of extracting time, 80 癈 of temperature, 2 times of extraction ,10.5 of pH value were the best extraction conditions of the BLF. At these conditions, the absorbency of extracting solution could reach 0.286 , the extracting rate was 90.59%.Then the purification of BLF was studied, and the optimal purification conditions were concluded that the macroporous adsorption resin ADS-17 was selected as exclusive separation resin of BLF, the adsorption velocity was 3mL/min, elution velocity was 1.5mL/min, diameter and height rate was 1:7.5, the pH value of under-adsorpted solution was 9, the elution solvent was 40% alcohol, and the quantity of elution solvent was 3BV( resin bed volume, its abbreviation is BV). Then the eluent which got from above conditions was dehydrogenized by vacuum freezing drying method, the tan powder of BLF was collected.Finally, the tan powder of BLF was evaluated. And so the conclusion were : the purity percentage of BLF was 28.04% , after adsorpted by ADS-17 resin and eluted by 40% alcohol, the purity percentage of BLF became 3.274 times of the pre-adsorpted solution, increased 2 times or more, the yield rate of BLF in product was 3.744%; The total saccharide content of product was 38.65%, and the content of the compound containing nitrogen of product(calculated as protein) was only 1.58%; the flavonoids of product detected by color reaction could belong to flavonol or flavone, the flavonoids in product detected by TLC could belong to flavone , the absorption spectrum of flavonoids in product detected by ultraviolet spectrometer had the characteristics of the absorption spectrum of flavone . So from above , it can bedetermined that the flavonoids in product probably was or were a kind or kinds of flavone.