Study And Application Of Molecularly Imprinted Technology In Drug Analysis

The opinion of molecularly imprinted polymer come from the recognition that antibody is single-minded to antigen, so is enzyme. Pauling, who ever had wined Noble prize, first planed synthesize antibody with antigen as template. Inl949, Dickey first experimented imprinting dye on silica gel. Until 1970s', people didn't succeed in imprinting molecular on organic polymer.Molecularly imprinted polymer can be prepared as follows: First, template molecular and functional monomers are mixed in an appropriate solvent to form complex; Secondly, cross-linking monomer is added into the solution; and the complex is cross-linked; and thirdly, subsequent removal of the template molecular leaves behind cavities complementary to the template molecular that are expected to work as selective binding sites. Interactions between molecularly imprinted polymer and template molecular mostly are ionic bond, hydrogen bond or metal complex. With the characteristic of predetermination, recognition and practicability, MIPs are used in many fields, such as chiral separation of enantiomer and isomer, clinical drug analysis, enzyme-minic catalyzed reaction, solid phrase extraction, chemical bionics sensor and film separation.With the direction of theory of molecularly imprinted polymer, this paper synthesize different polymer with template molecular and study several field of molecularly imprinted polymer in system.(1) Evaluation and recognition mechanism of molecularly imprinted polymer. With Benztriazole as template molecular, we synthesized molecularly imprinted polymer in accord with the technology of molecular imprint; studied its recognition mechanism through the model of Langmuir and Scatchard and discussed the effects of solvent polarity and ionic intension on recognition mechanism. We also studied the recognition mechanism of metal complex molecularly imprinted polymer.(2) Synthesizing conditions of molecularly imprinted polymer. Different syntheticalmeasures, various kinds and dosages of functional monomers, cross-linking monomer, initiator and solvent have different effects on the performance of molecularly imprinted polymer. With Mesylate as template molecular, we synthesized microsphere molecularly imprinted polymer with lots of solvent and discussed the optimal condition, which offered experimental measures for synthesizing best performance molecularly imprinted polymer.(3) Appliances of molecularly imprinted polymer. With the characteristic of predetermination, recognition and practicability, MIPs are used in many fields. With (s)-ketoprofen as template molecular, we synthesized a kind of molecularly imprinted polymer, which can separate racemic ketoprofen. With Zn-2,2'-dipyriyl complex as template molecular, we also synthesized a kind of molecularly imprinted polymer and discussed its selective recognition to different molecules.