Application Of Flow-injection Chemiluminescence In The Pharmaceutical Analysis

Flow injection (FI) chemiluminescence (CL) analysis has made great progresses in analytical chemistry for its high sensitivity with a wide linear dynamic range, simple instruments, rapidity and reproducibility in signal detection, and being appropriate for on-line analyses. This thesis focuses on the applications of FI-CL method in determination of drugs. The work develops a new FI-CL system for determination of uric acid, folic acid and rutin based on the chemiluminescence reaction of Rhodamine B-Ce (IV) in sulfuric acid media. The main content consists of four parts:Part I FI-CL analysis and analytical applications in determination of drugsThis literature review provides a simple introduction for the development of FI-CL analysis. The mechanism of CL reaction and the basic theory of FIA were discussed briefly. The research progresses of FI-CL method in determination of drugs since 1985 were discussed systematically.Part II Flow-injection chemiluminescence determination of uric acid in human urine by use of the Rhodamine B-Ce(IV) systemA highly sensitive flow injection (FI) chemiluminescence (CL) method has been developed for determination of uric acid based on its enhancement effect of the CL resulting from the reaction of Rhodamine B with Ce (IV) in sulfuric acid media, cationic surfactant CTMAB is used as a sensitizer. The sensitized-type of CL emission is linear over the the logarithm of uric acid concentration in the range of 1.0 #10-13 ~5.0# 10-11 mol l-1, with a detection limit (3σ ) of 3.7×10-14mol l-1. The experimental conditions for the reaction are optimized. The method is applied to the determination of uric acid in human urine with good accuracy and precision.Part III Flow-injection chemiluminescence determination of folic acid by use of the Rhodamine B-Ce(IV) systemA new FI-CL method for the determination of folic acid is described. The method isbased on the enhancement of chemiluminescence of Rhodamine B-Ce(IV)-H2SO4 system by folic acid. The optimum conditions for the CL emission were investigated. With peak height as a quantitative parameter, folic acid can be determined over the concentration range 3.0× l0-6~3.0×10-5 mol l-1 (r=0.9994, n=7) with a detection limit (3a) of 3.0 × 10-6mol l-1 original concentration. The method is successfully applied to the determination of folic acid in a pharmaceutical tablet preparation.Part IV Flow-injection chemiluminescence determination of rutin by use of the Rhodamine B-Ce(IV)- systemA novel FI-CL method has been developed for the determination of rutin based on the CL reaction of Rhodamine B with Ce(IV) in sulfuric acid media. The CL signal produced by the reaction is decreased in the presence of rutin. Under the optimal conditions, the CL intensity is linear to the logarithm of rutin concentration in the range of 3.0×10-6~1.0×10-4 mol l-1, with a detection limit (3σ) of 1 × 10-6 mol l-1. The method is applied to the determination of rutin in pharmaceucal preparations with satisfactory result. The possible reaction mechanism in the system is discussed briefly.