| Live Yeast Cell Derivative (LYCD), a kind of protective substance which could increase cellular respiration and wound healing ,was produced by living cell response to a controlled injure. LYCD was extensively used in cosmetic and pharmaceutical fields.This study was based on LYCD produced under oxidative stress, and the optimum condition to produce LYCD was discussed. Reduced glutathione(GSH) and superoxide dismutase (SOD), the two important ingredients in LYCD, were determined and analyzed, and the stress proteins in LYCD were studied through gel electrophoresis . In addition, LYCD produced under UV stimulation were also studied.The experiment suggested that 80 percent destroying rate could be reached by breaking up the yeast cell wall with liquefaction nitrogen, and Saccaharomyces cerevisiae TQ 22015 was selected as the best strain to produce LYCD. Through single factor and orthogonal design, the optimum conditions of producing LYCD were as follow: the concentration of H2O2 is 0.2 mmol/L, and the treating time is 15min.The following conclusions were drawn from the experiments: the specific activity of SOD in LYCD was strongest after the cell was treated with HA for 15min, while the quantity of GSH reached the highest at 30min. They showed different available time respectively.Through SDS-PAGE and two-dimensional gel electrophoresis experiment, the stress proteins in LYCD were analyzed. A dramatic change happened under HA stress: some new proteins were synthesized, while the syntheses of some other proteins were repressed.It was concluded from contrast experiments that no matter how they were produced, under H2O2 stress or UV stimulation, LYCD had almost the same biological activities. At the same time, the specific activities of SOD were higher than the control, and the quantities of GSH were increased also. The result indicated that these two stress responses were concerned with each other at some extant, though they might be produced through different mechanisms.
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