Research Of Tramadol Hydrochloride, Minocycline Pharmacokinetics And Moxonidine Stability Analysis

The application of HPLC in pharmacology advances very rapidly in our country in recent years, and the trend is still more and more powerful. It is forecasted to be one of the strongest analysis means in 21st century, especially in clinical parmaco-kinetics.The dissertation mainly consists of four aspects. 1 The stabilityof moxonidine capsule was studied, and the content of moxonidine capsule was determined by means of RP- HPLC. The samples were tested by accelerating experiment % room temperature storage and strong light exposure experiment respectively. Under the normal conditions moxonidine capsule is very stable , but when temperature is above 120#, moxonidine is decomposed, and the stability of capsule is destroyed. 2 A reversed-phase high performance liquid chromatography (HPLC) method was established to determine the concentration of tramadol hydrochloride in human plasma, and studied its pharmaco-kinetics. HPLC instrument was used with the column:150mm × 4. 6mm(Lichrosorb C18, 5μm). The mobile phase was composed of phosphate buffer solution (0. 02mol/L, pH=3. 7)-acetonitrile (83:17,V/V). Flow rate was 1.0mL/min. Detection wavelength was 216nm. The standard curve equation was Y=0.03244X+0. 6007, The linear range was 25~800ng/mL. The minimum detection limit was 10. 2ng/mL. Pharmaco-kinetics parameter for tramadol hydrochloride tablet were T1/2Ka=0.45±0.21h, T1/2 beta =5.85±2.48h, Tpeak=1.65 ±0. 52h,Cmax=348.60±98.65ng/mL The area under the curve of concentration versus time after 100mg oral of tramadol hydrochloride tablet was AUC0-=3245. 6±1516. 02(ng/mL).h. The reversed-phase HPLC method is simple, rapid and sensitive. It is applicable to determine the concentration oftramadol hydrochloride in human plasma. 3 Conditions have been studied forthe determination of minocycline in human plasma by RP-HPLC. Chromatographic separation has been achieved on C18 column with 0. 2mol/Loxalic amine: bimethyl-tetramine: 0.1mol/LEDTA(550:250:200) as the mobile phase. Detection was at UV 355nm. The drug and an internal standard were extracted in ethyl-acetate from buffered plasma(pH6.2). The standard curve of minocycline was linear in the range of 1?μg/mL. The minimum detection concentration was 0.2ng. Pharmaco-kinetics parameter for minocycline tablet were Tpeak=2.69±0.9111, (Cmax,=4. 45±0.95μg/mL. The area under the curve of concentration versus time after 100mg oral of minocycline tablet was AUC =32.45±15.16 (μg/mL). h. The reversed-phase HPLC method is simple, rapid and sensitive. It is applicable to determine the concentration of minocycline in human plasma.Moreover, we also have done some work on developing phenylketonuria (PKU) screening kit. The clinical experiments validated that it was simple, sensitive and stable. It is very convenient to extend in many common hospitals.