Studies On The Extraction And Anti-tumor Activity Of Total Flavonoid From Tephroseris Kirilowii (Turcz.) Holub.

The systematic analysis and anti-tumor activity were achieved through the extraction, determinant, quantitative and toxic test on the total flavonoids (TF) from Tephroseris kirilowii (Turcz.) Holub.1.The extraction of the total flavonoids (TF) from T. kirilowii The total flavonoids (TF) were extracted by cool and hot methanol methods from powder of T. kirilowii. The extraction rate of gum from the hot methanol method was higher than the cool leach method, but the content of the total flavonoids was lower. The cool leach method was applied in the experiment.2.The determinant test of the total flavonoids (TF) from T. kirilowii The total flavonoids (TF) from T.Kirihwii contain mostly flavones, flavonols, bihydroflavonols, isoflavones, etc. It concluded by the results of the color reaction of filter and the thin layer chromatography. The structures mainly contained de-5-OH flavones, 5-OH, 4'-OH flavones, 5-OH, 4'-OH, 3-OR flavonols, 4'-OH flavonols and 7, 4'-bihydro -xide flavonols.3. The quantitative test of the total flavonoids (TF) from T. kirilowiiThe result of colorimetric determination was 5.711% of the total flavonoids (TF) from T. Kirilowii by the spectrophotometry which mensurate on the wavelengh in 510nm with the rutin as mark and the distilled water as control. It could supply the basal theory for the next study.4. The LD50 determination of the total flavonoids (TF) from T. kirilowiiThe LD50 of the total flavonoids (TF) from T. Kirilowii was determined using female KM mice by intraperitoneal injection according to improved Karber's method. The LD50 was 1392.52+94.62 mg·kg-1 which belong to lower toxicity. Its toxicity evidently lower than the LD50 of the extraction from T. Kirilowii using 60% ethanol solution and the alkaloid monomer. It concluded that the pyrrolizidine alkaloids lead to T.Kirilowii poisoning in clinic. The flavonoids from T. Kirilowii were likely to have the anti-tumor activity.5. The effect the total flavonoids (TF) from T. kirilowii on three tumor cellsThe inhibit rates were determined by MTT colorimetry method to study the total flavonoids (TF) from T. Kirilowii on three tumor cells. The inhibit rates (IR) increased with the concentration heightening that they were positive correlation. The results showed that the different concentration of total flavonoids was low sensitive to liver-cancer Hep-G2cells. At the concentration of 10.0ug·m-1 TF was medial sensitive to liver-cancer HCC cells. At the concentration of 10.0ug·ml-1 and 100.0ug·ml-1 were medial and high sensitive to leukemia L1210 cells respectively. And the half of inhibit concentration (IC50) of total flavonoids on leukemia L1210 cells was 7.756ug·ml-1. These results suggest that the total flavonoids (TF) from T. Kirilowii have higher anti-tumor activity to the L1210 cells than the HCC and Hep-G2 cells.6. The studies of the total flavonoids (TF) from T. kirilowii on the L1210 cells in vitroThe rate of growth was slow and mPDT increased greatly (P<0.01 ) at the concentration of 100ug·ml-1 of TF by studied on the growth curve and mPDT of the L1210 cells. At the concentration of 100.0ug·ml-1 of TF, it showed high cytotoxicity and made the growth stagnancy. The result of the resist-dye rate of trypan blue showed that the different concentration of total flavonoids (TF) could all restrain greatly the activity of L1210 cells. The rate of lively cell was decreased with the concentration increasing. At the concentration of 10.0ug·ml-1 and 100ug·ml-1 of TF possessed better inhibition to the activity of leukemia L1210 cells (P<0.01). It indicated that the total flavonoids (TF) inhibit the activity of L1210 cells and the inhibition rate had a positive interrelationship with the concentration. The conformation of L1210 cell had round and assemble tendency by the total flavonoids (TF). The amount decreased and assemble severed with the increasing concentration. It had lots of fragment in culture liquid. At the concentration of 10.0ug·ml-1 of TF, the amount of S phase L1210 cell increased great...