Study On Interaction Of Drugs With Protein And Cells By Capillary Electrophoresis

A amount of work has been done in the field of molecular interaction analysis by capillary electrophoresis, and a great deal of achievement has been obtained. But in the study of interaction on the drug molecules with the bio-molecules or cells, the adsorption of proteins on the capillary wall and the background induced by the biological particles are serious. To solve the problems, the interaction analytical methods have been improved and developed in this thesis.The study of interaction between serum albumen and drugs is important in the investigation of the drug transfer and absorption in body which can be acted as assistant in the drug screening. The interaction analysis between cell and drug is important in the drug development and pharmacology. The paper is made up of two correlative parts. In the first part, the interaction between serum albumen and drugs were involved; in the second part, the interactions between red blood cell and drugs were studied.The studies on interaction between drugs and serum albumen were consisted of Two interaction systems with the partial filling-self marker technique. One was the study on the interaction between human serum albumen (HSA) and nicotine and the binding constant of them was 1.66×103mol-1L. Another system is the interaction between bovine serum albumen(BSA) and ephedrine, caffeine and acetaminophen, and the binding constants between BSA to them were got, to be 3.38 ×104 mol-1L, 4.79×104mol-1L and 6.13 ×104mol-1L, respectively. In the study, the partial filling with the fore plug self marker technique for CE was built, which successfully reduced the interference from the absorption of the protein buffer.In the second part, the partial filling -self marker technique was employed to study the interaction between the red blood cell (RBC) and ephedrine, caffeine and epinephrine and the interaction was evaluated by a parameter in the form of binding constant named as binding parameter. By the parameter, the interaction between cells and drugs can be described. The experiment fidelity was proved to be poor by the study of the ephedrine in different experiment. The comparing between the interaction between different drugsand BRC was advised to be done in a same experiment. The binding parameters between ephedrine and caffeine to RBC was compared and the difference between was explained. The binding parameters between ephedrine and epinephrine to RBC was compared and the difference between was explained in principle.The thesis indicated that the partial filling fore plug drug self marker technique is an effective method in the CE interaction study which has developed the peak-shifting model refined Scatchard plot and enlarged its scope. A way of uncovered capillary studying the interaction between protein and drugs was developed, which can not only be successfully used in the interaction study on of protein to drugs, but be used in the study of interaction between cells and drugs and a way to study the interaction between cells and drugs by CE was exploited.