The Process Research For Uridine 5'-monophosphate Separation And Purification

5'-Nucleotides include Uridine 5'-monophosphate(5'-UMP), Guanosine 5'-mono-phosphate(5'-GMP), Cytidine 5'-monophosphate(5'-CMP), Adenosine 5'-mono-phosphate(5'-AMP), They have wide use in the industry of food. medicine and fine chemicals, especially applying in the field of food and medicine, which are unrep laced.Presently companies inland that produce four kinds of 5'-nucleotides are very few, and the purity of products is not high, and yield is low. Every year we must import a lot of nucleotides, which can't meet the demand. The shortage of stuff has restricted the use and development of nucleotides in food, medicine and fine chemicals.From the viewpoint of industrialization of nucleotides, this article studied the production method of four substances taking advantage of cheap zymohydrolysis-ion exchange separation.Because the physical and chemical property of nucleotides is very similar, and there are many impurities in the solution of zymolysis, the separation of down stream is greatly difficult. At present inland the shortcoming in separation is that the resolution and use ratio of resin is low, the amount of coloring matter contained in products is too high, and the purity and yield of nucleotides is too low and etc.Aiming at above problem, this paper put forward the strategy of separation as follows. Firstly, in view of great amount of coloring matter in solution, the quality of product is declined. So we decolor the solution by acticarbon, which makes 80% coloring matter be removed, but the loss of nucleotides is only about 2%. Secondly, on the basis of physical and chemical properties of four nucleotides, we first synthesized a kind of cation exchange resin by ourselves which is used to separate four matters preliminarily. The advantage of this resin as follows. The adsorption capacity of resin toward nucleotides is quite high. The resolution of resin toward four matters is high. The yield of products is high. too. The average yield greater than 93%. Except the purity of UMP is lower than three others, the purity of GMP, CMP, AMP is all greater than 93%, and they can be concentrated and crystallize high quality products whose purities are greater than 98%. The amount of impurity in the solution of UMP is great, which is removed difficultly. We synthesizedseveral differently qualitative anion resin, and screened out an anion resin-SD3 which possesses high adsorption capacity, high separation factor for UMP, and high wiping off ratio of impurity. The adsorption capacity of resin to UMP is 0.133g/g.wet resin, the separation factor a UMP后杂 is more than 5.0, the wiping off ratio of impurity more than 45%, and the adsorption isotherm line f (c)<0 is favorable for adsorption equilibrium. From the kinetic behavior of SD3 adsorbing UMP, we learned that when the concentrations of UMP are 1g/L and 6g/L, the diffusions come under FDC and PDC respectively. At the same time, we researched roundly the dynamic process of SD3 adsorbing, eluting UMP, from which we learned several technological conditions of SD3 purifying UMP. All of these haven't been reported. Finally we preliminarily researched the crystallization technological conditions of UMP.This method possesses that procedure is simple, the demand for equipment is low, and the cost of production low, and is propitious to the demand of wholesale industrialization.