Studies On Extraction & Purification Processes Of Total Alkloids Of Eomecon Chinantha Hance

Studies on Extraction & Purification processes of Total Alkloids of Eomecon chinantha HanceMastership candidate:Huang songTutors:Prof.Du Fanglu ( Hunan College of TCM , Changsha, China,410007 )Eomecon chinantha Hance is a many-year living herb and a species of unique category and unique genus in China,extensively distributing in some southern provinces such as Zhejiang,Jiangxi,Sichuang,Hunan etc.Its root and rhizoma are used in medicine.In the traditional Chinese medicines,it can clear away hot and toxic. Eomecon chinantha Hance mainly contains Chelerythrine, Sanguinarine, Protopine,α-allocrypopine, Oxysanguinarine,Chelerythridimerine and other alkaloids.The content of Chelerythrine is the most.The modern pharmacological study indicates that the alkaliods can block cardiac cell passages and prevent various experimemtal virulentcite cardiacarrhythmia.It also inhibits the action of enzymes and WBC in human body and animal cells,fluences cell's fuction,and destroys bacterium and virus. Therefore,the aim of the study on the extraction and purification processes was to provide scientific basis for the manufacture of total Eomecon chinantha Hance alkaloids.On the bases of previous researches into Eomecon chinantha Hance andon consideration of specific resources of the laboratory ,this article optimized the parameters of reflux extraction which was screened by the orthogonal design.The purifacation prosess of total alkaloids with macroporous resin was generally studied by taking adsorption ratio ,eluting ratio and purification of total alkaloids as indices.The results were as follow:1.The optimal parameters of the reflux extraction were that:Eomecon chinantha Hance refluxed in the 95%EtOH(6-folds) for 1.0h ,3 times in all.2. The study of purification process showed that AB-8 macroresin had themaximum adsorption ratio among three resins:D101A,D101B and AB-8. Its dynamic and static state adsorption ratio were : 3.229±0.012mg.g-1 dried resin, 3.750±0.024mg.g-1 dried resin; the elution ratio was 93.13±0.21%. The experiments showed the optimal parameters of purification were that:original concentration(0.4-0.6mg/ml) ,PH value (7.0-8.0), velocity of flow (3BV/h), then eluted by water and 95%EtOH, gather the part eluted by 95%EtOH, and total alkaloids in the total solid extraction could reach 50.39%, the refine ratio could reach at 596.26%.The results of the whole experiment has provided the basic data forpreparation process and quality control of modern Chinese pharmaceuticals intend for the treatment of hypertension,which was a referance for process improvement and quality control of similar Chinese herbal pharmaceuticals as well.In the study ,the application of macroporous resin primarily demonstrated that these new techniques and new materials could enhance extrating and purifying ratios of effective components in Chinese herbs,It will be feasible to promote their application to Chinese herbal pharmaceuticals R&D...