Study On The Supramolecular System Of Porphyrin With Cyclodextrin, DNA And Protein

In this thesis, electroanalytical method of Tetrakis(4-carboxylphenyl) porphyrin(TCPP)has been established. In a supporting electrolyte of KH2PO4-Na2HPO4 (pH 7.0) , a sensitive second derivative reduction peak of TCPP was found by single-sweep oscillopolarography. The potential peak is -0.70V(vs.SCE).The relationship between peak height and the concentration of TCPP is linear from 1× 10-7 to 2×10~5mol l-1,the limit concentration is 6× 10-8mol/L.The relative standard deviation (RSD) was 0.41%(n=8), and the recovery of TCPP varied from 95.8% to 105.4%.The Supramolecular system of cyclodextrins (CD) with TCPP in NH3-NH4C1 (pH 8.0) has been studied by polarography. The TCPP can form the 1:1 inclusion complex with β-CD,γ-CD, Hydroxylpropyl- β-CD, Sulfurbutylether- β -CD and Trimethyl- β-CD. "current method" has been used to determine the formation constants of TCPP with five CDs. The result shows that the inclusion ability of Hydroxylpropyl- β-CD is very strong. Moreover, modified β-CD has stronger inclusion capacity than native β-CD. The formation constant of TCPP with γ-CD is much greater than that of TCPP with β-CD, because the γ-CD has a bigger cavity that can match with the size of the meso-phenyl of TCPP.The Supramolecular data will provide useful information for further application of TCPP;The Supramolecular system of two water-soluble porphyrins (TCPP, TMPyP) with SBE-β-CD has been studied by UV spectroscopy and Fluorescence. The result shows that bothTCPP and TMPyP have formed 1:1 inclusion complexes with SBE- β-CD,and their formation constants are 1.34 × 104 and 1.15 × 105Lmol-1',respectively.Their possible inclusion mechanism has been discussed; The interaction of TMPyP and its metal complexes with ctDNA has been investigated by means of UV-Vis spectroscopy and polarography. The experimental data show that TMPyP and its metal complexes can form stable complexes with ctDNA, and their formation constants have been determined by current method. The result indicates that the interaction of TMPyP and its metal complexes with ctDNA surely exists by electrostatic attraction; The Supramolecular system of TCPP with BSA has been probed by UV-visible absorption spectroscopy, polarography and fluorescence. The electroanalytical method of BSA has been established. The relationship between peak height variation of TCPP and the concentration of BSA is linear from 2×1.0~6tol×10~5mol/L,the limit concentration is 1.2×10~6mol/L.the relative standard deviation (RSD) was 4.0%(n=8), and the recovery of TCPP varied from 95.0% to 104.1 %.amino acids which are as 50 times much as BSA did not interfere with the determination of BSA, and some metal ions (Co"+> Ca24\ Cr \ Fe3+) which are 20 times more than BSA will interfere with the detection of BSA. Moreover, the formation ratio and formation costants between TCPP and BSA have been calculated by the above methods. The experimental data show that TCPP and BSA can form 1:1 complex by hydrophobic effect.