The Study Of Disruption Effects Of Environmental Endocrine Disrupting Chemicals In Blood

Environmental Endocrine Disrupting Chemicals (EDCs) is defined as an exogenous substance that presents in the environment and causes adverse health effects in an intact organism, or its progeny, consequent to changes in endocrine function.With the development of EDCs research, more and more chemical materials are considered as "potential endocrine disrupter" which might be expected to lead to endocrine disruption in an intact organism. In order to develop the knowledge of EDCs' harm and control it more efficiently, research of the interaction mechanism between EDCs and organism has been valued by more and more researchers.Estradiol, testosterone are two hormones in human body, and 2,4-D , Atrazine are considered as two kind of EDCs. The interaction between estradiol, testosterone, 2,4-D, Atrazine in BSA or human serum were studied by fluorescence analysis. The results show that both hormones and EDCs have a strong ability to quench BSA or human serum fluorescence, which is mainly through a static quench procedure. The binding constant K and the number of binding site n were calculated according to the fluorescence quenching expressions, and the difference between hormones and EDCs in organism was compared.In order to develop the interaction mechanism between EDCs and organism, the interaction between hormone and EDCs in hormone/EDCs/BSA (human serum) ternary system was investigated by fluorescence measurement. The binding constant K was calculated with Lineweaver-Burk method, and the influence between hormone and EDCs on binding constant K was obtained. Consequently, endocrine disrupting mechanism of 2,4-D and Atrazine was concluded.