The Development And Application Of UV Curing Coating For SPME

At first, this paper introduces solid phase microextraction ( SPME ) technology , includes studies of course parameter applications in the pretreatment of the sample, the prospect of study and the theoretical foundation,. Then, this paper introduces the work of this experiment including the following several respects:(1) Assemble a simple device for SPME with a micro- syringe, and set up the system for appraising the enrichment ability of SPME coating to solid sample and liquid sample. Through the comparison of several appraisal system, choose direct SPME method for main appraisal system because this system is relatively easy to be kept for standard solution, fast to be analysed and easy to get the instruments to use.(2) In order to optimize the condition of analysis for the chosen direct SPME system, the chosen components are: Chloroform, acetic acid, n-Amylalcohol, n-butyl acetate, DMF, p-xylene, cyclohexanone, aniline, Phenol. The chromatogram conditons chosen are: Column: (30m×0.25mm×0.25μm, UA-17), Carrier gas: N2, 0.1 MP. Detector:FID, H2,0.1MP; Air:0.1MP. split: off.Column temp : 40℃ (1 min ) to 100℃ (2 min) at 8℃ /min. Injector temp: 220℃, Detector temp:250℃. Under this condition, the linear range , the limits of detection , the recoveries, the relative standard deviations of the method to every component have been carried on.(3) Have been developed new-type SPME coating by the commonly used ultraviolet (uv) curing coating, has offered a kind of simple and convenient and fast method to be suitable for laboratory to make SPME coating by oneself . Through optimizing the liquid UV-curable composition (kind of oligomer, concentration of active diluent , photoinitiator and additive), we have got a kind of better ingredient , as follows: PUA 100% and additive (TF, AB and nanometer additive MNIP0220) 100%.(4) Compares HS-SPME (made of above ingredient) with routine HS method under the identic measuring condition. Take pepper for example, the area of responsing peak of main component is 62052680 in HS-SPME and 38263 in HS(10u1), a enrichment multiple of 1622, which is proved that this SPME coating has a better enrichment ability. (5) Compare self- SPME apparuts ( coating volume is only 1/4 offoreign products) with foreign SPME products - Supelco SPME( PA) , under the same analysing condition, earring on HS-SPME-GOMS for comparing, it can been seen by the result, as to some composition, the absorbing amount of self-SPME apparatus has at least one time higher than that of SPME( PA) of Supelco Company. Generally speaking, self-SPME apparatus and SPME( PA) of Supelco of Company have equal enrichment basically.(6) Compare direct SPME with direct inject, the SPME apparuts that is made by this experiment has the enrichment rate of 51 in P-Xylene, higher than the enrichment rate of SPME apparuts from the document.(7) Analyzed volatile chemical composition of two kind of herbs with HS-SPME" GC-MS. The results are satisfactory, proving that adopting self-SPME apparatus can be succeed in enrichment of volatility composition and used in the microanalysis of the volatile matter.