The Production Of Lignin Peroxidase By Phanerochaete Chrysosporium And The Decolorization Of Ddyes By The Immobilized Lignin Peroxidase

In this thesis ,we first study lignin peroxidase production by Phanerochaete chrysosporiun on porous polyurethane foams . On the basis of this , we studied crude separation and purification of lignin peroxidase , immobilization of the lignin peroxidase and the degradation of the dyes by the immobilized lignin peroxidase.From our investigation , we found that effection of lignin peroxidase production by the Phanerochaete chrysosporiun immobilized porous polyurethane foams are good . Among the five kind of on porous polyurethane are tested , the third is the most effective in the fermentation experiment.The mycelium immobilized stablely in the foam of the following and fermentation liquor was clear, which is beneficial on the post-treatment, Polyurethane foam is a suitable carrier for its cheap price and effectiveness.We optimize three parameters which are the most effective in the lignin peroxidase production through orthogonal method : the amount of the carrier , the amount of ammonium tartrate and the the rotation. The optimal value of the three parameters are 4g/L, 2g/L and 190r/min respectively. Under which the production was increased and the activities of the lignin peroxidase can reach 200U/L Additionally , inoculation amount test show that it influence little in the fermentation.Repeated batch fermentation of the immobilized Phanerochaete chrysosporiun indicated that we can get the lignin peroxidase if we adding initial fermentation medium or adding medium which is lower in glucose or ammonium tartrate, We can reused the immobilized mycelium and bypass the incubation period after immobilization. Furthermore , reducing glucose or ammonium tartrate is an economic way in reducing production cost.We did some crude separation and purification of the lignin peroxidase.In the research we found that the concentration of 45% ammoniumsulfate is the best when precipitation of the lignin peroxidase, the acquirement rate of which is 86% , We optimize three parameters that are the most effective in the activity of immobilized lignin peroxidase production through orthogonal method : The optimal value of the three parameters are 3% sodium alginate, 0.4% glutaraldehyde and 0.2mol/L Calcium Chloridere respectively, under which the spectrums of the optional pH and optional temperature were expand and had a better stability.When the four kind of dyes were degraded directively by the enzyme solution. They all had different extents of the degradation . In the test of using immobilized lignin peroxidase degrade dyes , We chose disperse Rubine as the object and investigated the potential of our immobilization system. In the comparison of the decolorization results of the three concentrations of the disperse Rubine we tested , we could conclude that the decolorization rate of the higher concentration are lower than those of the lower concentration..The result indicated that the immobilized lignin peroxidase could be reused at least of 7 times. In the test of the decolorization we found the colorization rate is declined gradully which due to the reducement of the concentration of H2O2.