Preliminary Studies On Decolorization Or Degradation Of Triphenylmethane Dyes And Bamboo Alkali Lignin By Ligninolytic Enzymes

Triphenylmethane dyes and Lignin are aromatic compounds that difficult to degradate in nature. These compounds continue to accumulate that caused severe environmental pollution. In nature, White-rot Fungus degradated effectively to these compounds depended on special enzymes, which mainly includes ligninolytic enzymes (lignin peroxidase, mangnase peroxidase, laccase). So that, triphenylmethane dyes and lignin degradation by white-rot fungus was hot spot in biotechnology research.In this dissertation, mangnase peroxidase (Mnp) and laccase (Lac) were research object which producing strains are Phanerochaete chrysosporium or Pleurotus ostreatus. Preliminary studies on the decolorization and degradation of triphenylmethane dyes or lignin by mangnase peroxidase and laccase, and the synergy of two ligninolytic enzymes were studied.In this paper, the ability of producing Mnp or Lac by white-rot fungus was researched in the early study. The results showed that, Phanerochaete chrysosporium strain inoculated in the enzyme selective production fermentation medium, then shaking cultured ten days, the enzyme activity of Mnp was 4.22 U/mL, and increased the inoculation amount, the enzyme activity of Mnp will increase to 6.51 U/mL. However, Pleurotus ostreatus strain inoculated in the potato juice medium fermentation medium, then static cultured twelve days, the enzyme activity of Lac was 4.56 U/mL, and increased the inoculation amount, the enzyme activity of Lac will increase to 7.91 U/mL.?Secondly, the effect of temperature and hydrogen ion concentration to? enzymology characters of ligninolytic enzymes were researched. The result showed that, the optimum temperature of Mnp was 40℃, and the optimal pH was 4.0, and the optimum temperature of Lac was 50℃, and the optimal pH was 6.0.Three kinds of triphenylmethane dyes, malachite green, bromophenol blue and crystal violet were tested for researching the degradation in the Mnp, Lac degradation system or Mnp-Lac degradation system. The results showed that, first of all, Mnp and Lac was the enzyme that will degraded the triphenylmethane dyes. Secondly, the molecular structure was the factor to affect the decolorization of triphenylmethane dyes. In addition, two ligninolytic enzymes have?the?synergy in the degradation process of triphenylmethane dyes. Alkali lignin was tested for researching the degradation in the Mnp, Lac degradation system. Our result showed that, alkali lignin had removaled by ligninolytic enzymes systems of white-rot fungus fermented, and the Lac system of Pleurotus ostreatus had the better removal rate than Mnp system. Finally, the results confirmed that Mnp and Lac had the degradation and flocculation to alkali lignin by UV spectrometry and GC-MS test.