| Chapter 1: The progress of DNA probe had been reviewed, including the kinds of DNA probes, the binding mode of DNA probe to DNA, the methods applied in this field and the applications of probe in DNA study; the progress of protein spectral probe had also been reviewed, including the kinds of protein spectral probes and the spectral analysis methods applied in the study on the interaction of the probe and protein.Chapter 2: The luminescence behavior of diiodofluorescein and tetrabromofluorescein had been investigated including the solid surface room temperature phosphorescence (SS-RTP) and the room temperature fluorescence (RTF). The luminescence intensities of the two compounds were strongest in alkaline solution. RTP lifetime of the two compounds are in the range of 130-140ms. The RTP and RTF polarity was in the range of 0.01-0.05. The two analytical methods-SS-RTP and RTF, of the two compounds had been established.Chapter 3: The interaction of tetrabromofluorescein (TBF) and DNA was studied by the fluorescence and SS-RTP. Deduced from the fluorescence and SS-RTP, there were two binding mode in the whole interaction: intercalation binding and groove binding. The benzopyranyl ring of the TBF intercalated between the ctDNA base pairs supported by potassium ferrocyanide quenching study, and the side chains of the benzene ring extended along the groove of DNA supported by phosphorescence polarization experiment. The effect of ethanol on the fluorescence intensity was smaller in the presence of DNA, compared with that in absence of DNA. The ion strength influenced the binding of TBF and DNA.Chapter 4: The fluorescence and solid surface room temperature phosphorescence (SS-RTP) spectral properties of tetrachlorotetrabromofluorescein (TTF) were investigated. And the factors influencing the fluorescence and phosphorescence emission were discussed. The interaction of TTF and ctDNA was studied by the fluorescence and SS-RTP. The fluorescence and phosphorescence emission changed by the addition of DNA.TTF intercalated into the ctDNA helix supported by fluorescence polarization experiment and potassium iodide quenching study. The ion strength can influence the interaction of TTF and DNA. Based on the fluorescence quench experiment of TTF by ctDNA, the binding constant of TTF binding to ctDNA was 2×106L/mol, the binding site was 0.16.Chapter 5: The binding of tetraiodofluorescein (TIF) to Bovine Serum Albumin (BSA) inaqueous was studied by Fluorimetry. The binding constant was 5.7×104L/mol, and the binding sites were 0.86.The research results of binding model indicated that the hydrophobia force was the main binding force. The presence of BSA could increase the fluorescence intensity of TIF. And the AF was linear to the concentration of BSA in certain range and the first analytical application was proposed. The linear dynamic range (LDR) was 8×10-7mol. L~9× 10-6mol. L, the limit of detection (LOD) was 9.88×10-8mol/L, the relative standard deviation is 3.4%.Chapter 6: The spectral properties of Lissamine Rhodamine RB-200 (LSR) were investigated, including fluorescence and SS-RTP. The excitation and emission wavelength of LSR is 568nm/586nm for fluorescence, and 570nm/702nm for phosphorescence. And the factors influencing the fluorescence and phosphorescence emission were discussed. The emission wavelength of the two compounds were located in longer wavelength which will avoid the interference of the bio-samples. The first analytical method was proposed by SS-RTP. The interaction of LSR and DNA was studied by absorption and fluorescence spectroscopy. The absorption and fluorescence intensity of LSR decreased by the addition of DNA. LSR intercalated into the DNA helix supported by fluorescence polarization experiment and potassium ferrocyanide quenching study. The ion strength influenced the interaction of LSR and DNA.Chapter 7: The fluorescence and SS-RTP spectral properties of methylene violet (MV) were investigated. And the factors influencing the phosphorescence emission were discussed. Th...
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