Electrochemical Studies Of The Protein And Chitosan With Organic Dye And Its Application

Protein is one of the most important component in the organism .It is closely related with nutrition, immunity, metabolism, origin and evolution of the life. Its level can well reflect the status of motabolize and immunity and can be as the important index of diagnosing ill, so the qualitative and quantitative analysis of protein is an important facter in biochemical,clinical and chemical assays .The chitosan and its derivatives are the major life actived substance, they can be used in anticancer ,antiruor, antithrombus and reducing the level of fat in blood. Recently, more and more people have focused on the studies of the protein ,chitosan and their derivatives. A great deal of methods about proteins determination have been developed, such as spectrophotometry on organic dye and organic dye-metal complex interaction with protein ,fluorometry and resonance rayleigh scattering (RRS).But the report about about electrochemical methods for the study interaction of small organic molecules with biomacromolecules has seldom been seen.The main contents of this paper are studies of small organicmolecles with biomacromolecles has seldom been seen.The main contents of this paper are studies of small organic molecules interaction with protein and chitosan by electrochemical methods, they are list as fellows:(1) Electrochemical study of thoron I interacting with bovine serum albumin(BSA)Thoron I can react with bovine serum albumin to produce an electrochemically non-active supermolecular Thoron I -BSA in the buffer solution of NaAc-HAc(pH=4.8). Thoron I has well-defined first order derivative linear-sweep polarographic wave at -0.58V(vs.SCE).Adding BSA into the Thoron I solution resulted in the decrease of the peak current and without the change of peak potential. The decrease of the, peak current is proportional to BSA concentration from 1.5 to 25mg/L,the limit of the detection is lmg/L,and thecorrelation coefficient is 0.998.This method can be applied to determine BSA concentration.(2) Electrochemical Study of Pr(III)-ALC-F~ Interacting with Bovine Serum Albumin(BSA)Pr(IH)-ALC-F" complex can react with bovine serum albumin to produce an electrochemical ly non-active supermolecular Pr(III)-ALC-F~BSA in the buffer soltion of (CH2)6N4(pH=4.9). Pr(III)-ALC-F~ complex has well-defined first order derivative linear-sweep polarographic wave at -0.66V(vs.SCE).Adding BSA into the Pr(III)-ALC-F" solution resulted in the decrease of the peak current and without the change of peak potential.The decrease of the peak current is proportional to BSA concentration from 1 to 17.5mg/l,the limit of the detection is 0.3mg/l,and the correlation coefficient is 0.998, this method can be applied to determine BSA concentration.(3) Studyies of Alizarin red S Interacting With Chitosan by Electrochemical methodAlizarin red S(ARS) can react with chitosan(CTS) to produce an electrochemically non-active supermolecular ARS-CTS in the buffer solution of NaAc-HAc(pH=5.0).ARS has a well-defined second order derivative linear-sweep polarographic wave at -0.408V(vs.SCE).Adding CTS into the ARS solution resulted in the decrease of the peak current and without the change of peak potential.The decrease of the peak current is proportional to CTS concentration from 0.5 to 7.5mg/L,the limit of the detection is ().4mg/L,and the correlation coefficient is 0.999. this method can be applied to determine CTS concentration,...