| Because of inherent high sensitivity, simplicity, a broad range of analytical applications with simple instruments, chemiluminescence(CL) has been received considerable attention in several analytical fields, such as bioengineering, pharmacology, molecular biology, clinic medicine, and environmental chemistry. When combined with Flow-injection it becomes a more effective trace analytical method. However, there are sill some problems when it is applied to the practical application due to its poor selectivity. When performed in reversed micells media, however, selectivity and sensitivity of CL can be improved in certain degree. The thesis consists of two parts. The first part is a brief review of the development of Chemiluminescence combined with other techniques which can improve its selectivity since 1994. The second part is the research reports in which the Chemiluminescence characteristic in the reversed micelles media is investigated and has been applied to the determination of the dissolved oxygen and five drugs, namely, chlorpromazine hydrochloride, promazine hydrochloride, quinine sulfate, berberine hydrochloride and methylenum blue in detail.1. Reverse Mcelle-based Chemiluminescence Determination of Quinine sulfate in Beverages and Biological FluidsA new chemiluminescence(CL) method is proposed for the determination of quinine sulfate, which is based on the dichloromethane solvent extraction of ion-pair complex of tetrachloroaurate(III) with quinine sulfate and luminol CL detection in a reversed micellar medium formed by the cation surfactant cetyltrimethylammonium bromide in a dichloromethane-cyclohexane (3:7 v/v) -water (0.35 mol/L NaiCO3 buffer solution with the pH of 11.5). The ion-pair complex of tetrachloroaurate(III) with quinine sulfate produced an analytical Chemiluminescence signal when it entered the reversed micellar water pool, hi the optimum conditions, CL intensities are proportional to concentrations of the studied drug over the range 0.015~10μg/mL with a detection limit of 1.5 ng/mL. The relative standard deviation (RSD) is 1.38% for 2.5μg/mL quinine sulfate (n=11). The method has been applied to the determination ofstudied drug in beverages and biological fluids with satisfactory results.2. Ion-pair Complex-based Solvent Extraction Combined with Chemiluminescence Determination of Chlorpromazine Hydrochloride and Promazine Hydrochloride withReverse MicellesBased on the CL reaction between AuCLT carried by the ion-pair complexes formed by the chlorpromazine hydrochloride or promazine hydrochloride and luminol in a CTAB reversed micellar medium. Chlorpromazine hydrochloride and promazine hydrochloride were detected. In the optimum conditions, CL intensities are proportional to concentrations of chlorpromazine hydrochloride and promazine hydrochloride, over the range 0.05-10 ug/mL and 0.05~5 ug/mL with detection limits of 5 ng/mL and 4 ng/mL; respectively. The relative standard deviation (RSD) are 2.6% and 1.4% for 1.25 ng/mL chlorpromazine hydrochloride and for 2.5 ng/mL promazine hydrochloride (n=ll); respectively. The method has been applied to the determination of studied drugs in tablets, injection and biological fluids successfully.3. Chemiluminescent Determination of Berberine in Tablet Form Based on Ion-pairComplex-solvent Extraction with Reversed MicellesA simple and sensitive method for the determination of berberine is described. The principles of ion-pair formation of berberine-metal complex and liquid-liquid extraction are applied to the chemiluminescent determination of berberine. On mixing with a reversed micellar medium of cetyltrimethylammonium bromine in a dichloromethane-cyclohexane (3:7 v/v) -water (0.4 mol/L NaiCO3) containing luminol, the ion-pair complex of tetrachloroaurate(III) with berberine produced an analytical chemiluminescence signal when it entered the reversed micellar water pool. Using the chemical conditions optimized for berberine in aqueous samples, a detection limit of 2x10~3 μg/mL was achieved...
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