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Isolation And Application Of High Solution Microbes For Animal Dung

Posted on:2005-10-14Degree:MasterType:Thesis
Country:ChinaCandidate:Y LingFull Text:PDF
GTID:2121360122993832Subject:Environmental Science
Abstract/Summary:PDF Full Text Request
In the past few years the "shopping basket program" has made our life become better and better, but at the same time, the discharge of animal dung has also increased quickly. There was 2.485 billion ton animal dung in 1995, which was 3.9 times as much as the industrial solid wastes. Now the animal dung has become one of the three main sources of water pollution, together with industrial waste water and daily life waste water. At the same time, researches on how to deal with animal dung pollution are being carried out and developed. By isolating, purifying and screening a batch of microbes from various separate sources, we have got high effectual complex inoculants from the animal dung, which are used in factory production and achieved good results. The results of this research are as follows:1.Using some kinds of selective media, we have got many kinds of mesophilic microbes and thermophilic microbes, including 26 strains of mesophilic bacteria(B), 9 strains of thermophilic bacteria(HB), 26 strains mesophilic actinomyces (A), 6 strains of thermophilic actinomyces(HA), 16 strains of mesophilic moulds (M) and 1 strains of thermophilic moulds (HM).2. Through the hydrolytic tests of four kinds of macromolecular organic matters, including starch, fats, protein and cellulose, 59 strains of microbes having strong degradation ability have been screened from the various microbes, including 15 strains of mesophilic bacteria(B), 4 strains of thermophilic bacteria(HB), 22 strains mesophilic actinomyces (A), 6 strains of thermophilic actinomyces(HA)and 12 strains of mesophilic moulds (M).3. Through the composting experiments, a group of complex inocula has been obtained, which can increase the compost temperature to 60 C in two days, accelerate the elimination of toxic gases and make the compost mature in three weeks. The inocula include 9 strains of bacteria and 4 strains of actinomyces.4. Temperature, water content rate, organic matter, NH3-N, proteinase activities and the numbers of the microbes have been studied in the next composting experiment. Significant correlations have been confirmed among temperature, water content rate and organic matter. Correlations exist in other parameters. After 14 days, it has been found that within the same time period, the temperature of the compost with the inocula is 4% higher than that of the control; the reduction of the organic matter is 3% higher than that of the control, the concentration of the NH3-N is 5% lower than that of the control, and the microbial quantity is 10 times more than that of the control. So high effectual complex inoculants have some good effects on thereduction of organic matter, the rise of the temperature and the efficiency of the composts.5. Partial physiological and biochemical indexes of the 9 strains of bacteria are measured. 7 strains of bacteria are Bacillus', 2 strains of bacteria are Micrococcus. The measurement of the growth curve shows that 7 strains of bacteria can reach a higher concentration of incubating within 48 hours. They are fit for industrial production.6. It is confirmed that the temperature of the compost can rise to 55 C in two days and remain more than 55 C in 12 days in producing organic fertilizer with our inocula in the factory. It can be concluded that the mature period of the compost with our inocula is one week quicker than that of the control because the parameters of this compost such as organic matter, water content rate, total nitrogen and NH3-N have become stable and the germination index value is over 50% in a month while the control need one more week to reach that condition. The compost's quality is also higher than that of the control because of the higher microbial quantity.7. By studying some indexes which are related to the compost maturity, the physical index, germination index, WSC/NO(w), NH3-N and the microbial quantity are suggested as the standards of the compost maturity, to solve the problem that a simple standard can not evaluate all composts, which gives a reference to the judgment o...
Keywords/Search Tags:animal dung, compost, complex inocula, organic fertilizer, bacteria identification, compost maturity
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