Study On The Function Of Mucopolysaccharide In Shark Cartilage And The Scheme Of Its Extraction And Purification

Mucopolysaccharide was a kind of natural molecular, which was basically consisted of aldoses linked by indicant bond, and was almost found in all organisms including animal, plant and microbe. Mucopolysaccharide was of extensive physiological function and was widely used for the purpose of antivirus, anticoagulation, antiulcer, antiinfection, blood sugar decreasing and lipid decreasing research. In this paper, mucopolysaccharide in shark cartilage was extracted and separated and the optimum extraction scheme and isolation method were screened. The results indicated that the harvested rate of mucopolysaccharide by using 0.3mol/L sodium hydroxide under 60-70℃ was relatively high. Negative ion resin D-241 combined with diehlaminoeth could extract mucopolysaccharide desirably and the result can be bettered by repeating diehlaminoeth one or two more times.In addition, the function of mucopolysaccharide from shark cartilage was examined in this paper. And the research mainly focused on its activity in antitumor and antisenity. The antitumor activity of mucopolysaccharide from shark cartilage was studied in vitro culture. Leukemia K562 cells in logarithm growth stage were made into suspension and cell density was adjusted to 5X 104 cells/ml. They were incubated in 24-well plate and 100μlcell suspense per well. Two concentration levels were set to examine its antitumor activity and the results were checked after 72h culture. Experimental results suggested that mucopolysaccharide from shark cartilage could inhibit the growth of Leukemia K562 cells to some degree. There was some correlationshrp between inhibition effect and mucopolysaccharide concentration and the inhibition effection of 5% mucopolysaccharide was relatively greater than that of 2.5% mucopolysaccharide. Viability of the cells was measured by a trypan blue exclusion test. The results indicated that viability of Leukemia K562 cells was reduced greatly by mucopolysaccharide treatment and mucopolysaccharide could inhibit and kill Leukemia K562 cells to some degree.Drosophilae was used as a model animal to study antisenity activity of mucopolysaccharide from shark cartilage. The experimental results suggested that the longevity and maximum longevity of Drosophilae treated by mucopolysaccharide could be elongated by 5.12 to 6.65 days and by 5.4 to 6.17 daysrespectively. The protein from Drosophilae head was analyzed by electrophoresis. Protein electrophoresis results indicated that compared with control, some protein of Drosophilae has significantly changed during the course of decrepitude, which may be caused by the expression of some gene. The possible mechanism of antisenity of mucopolysaccharide from shark cartilage was also discussed in this paper. Amylose environment in drosophilae's body may inhibit or retard the expression of some gene related to decrepitude. On the other hand, the immunity ability of organism was improved which may be favorable to resist adverse environment stress in body and out of the body. There was certain difference in the effecteion of mucopolysaccharide between male and female drosophilae.