Extraction And Research Of The Chondroitin Sulfate On The Function In Blood Lipid Reduction

The technology of extraction and purification of chondroitin sulfate were optimized by using chicken cartilage as raw material, then studied whether chondroitin sulfate has the effect of antioxidation or the function of reducing blood lipid.The chondroitin sulfate yield was used as indicators to determine the optimum extraction method of chondroitin sulfate from chicken cartilage was ultrasonic assisted alkali-double enzymatic. Then, optimized the three extraction conditions of enzyme dosage, enzymolysis temperature and enzymolysis time by using the Box-Behnken central composite design to establish the second regression model. Finally, we determined that the optimum conditions of the ultrasonic assisted alkali-double enzymatic extraction method of chondroitin sulfate were as follows:alkali concentration was4%, ratio material to solvent was1:6g/mL, Na+concentration was2.5mol/L, extraction temperature was35℃, papain dosage was1.3:1000g/mL, pepsin dosage was1.39:1000g/mL, hydrolysis temperature was47.5℃, hydrolysis time was102.5min, and the yield of chondroitin sulfate was28.67%, the purity was23.6%.The anion exchange resin and gel chromatography method was used to purify chondroitin sulfate. The anion exchange resin D218was selected to purify chondroitin sulfate by the way of static adsorption and analysis experiments. The best purification conditions of chondroitin sulfate was determined through dynamic adsorption and analysis was as follows:The rate of adsorption amount of liquid and resin volume was1:1, the adsorption velocity was1.0mL/min, the adsorption time was60min, the concentration of NaCl solution was3mol/L, the amount of eluant and resin volume ratio was5:1, the analysis time was75min, the purity of chondroitin sulfate was72.03%. Sephadex G75was selected to petrify chondroitin sulfate again, the optimum conditions of gel chromatography method for purificating chondroitin sulfate were as follows:the pH value of the acetic acid-sodium acetate was4.8, the elution velocity was0.7mL/min, the purity of chondroitin sulfate was99.30%under this condition, compared with the first purification improved27.27%.Sulfuric acid-carbazole method was used to detect the content of the extract from chicken cartilage. Detecting the substances of purification by gel chromatography through UV, HPLC and IR to confirm the extract from chicken cartilage was chondroitin sulfate.Study on the antioxidant activity of chondroitin sulfate extracted from chicken cartilage. The result indicated that chondroitin sulfate had the strong elimination ability to the five free radicals. Total antioxidant ability, the maximum inhibition rate to Anti-superoxide anion, hydroxy free radical, and Anti-liposome was30.56U/mL,97.74%,80.69%and48.05%. The maximum inhibition rate to Alkyl radical was78.57%. Studied the functions of defat of chondroitin sulfate extracted from chicken cartilage. The results indicated that CS could decline the level of TC、TG、LDL-C, and increase the level of HDL-C with the increment of CS.