Initial Study On Extract Craft And Pharmacodynamics Of Depurated Shanhejiangzhi Recipe

Hyperlipoidemia (HL) has been defined as one or more kinds of lipids are higher than normal standard in blood plasma due to lipids abnormal metabolism and transfer. It consists of hypercholesteremia, hypertriglyceridemia or both of them. It is associated with many diseases especially atherosclerosis and coronary artery disease (CAD). At present, many kinds of medicines are designed for controlling HL, whereas the results are limited. On the other hand, Chinese medicine pharmaceutics with many advantages such as affirmative therapeutic effects, prescription agilities, few side effects, low development costs and food-like dosage form is promising in new medicine development for HL.Objective:To study the best extract (EXT) craftflow-sheet of the depurated Shan-he-jiang-zhi (SHJZ) recipe; to evaluate the therapeutic efficacy on the rat model with Hyperlipoidemia made by the upgraded Shan-he-jiang-zhi recipe.Methods: (1) By means of the content of the crataegus total flavonoids as the indicatrix, inspecting the four different extracting method with Soxhlet extraction, ultrasound extraction, refluxing extraction and decoction extraction, further more investigating the depurating craft of the Crataegus by macroporous resin adsorptive process.(2)By means of the content of the lotus leaf total alkaloids and the yield of the xero-mastic as the indicatrix, inspecting the three different extracting method with acid-water percolation method ,alcohol refluxing extraction and acid-water refluxing extraction; further more comparing the organo-solvent extraction(Sx) with the method extracted by alcohol and pepted by water and ion-exchange resin separation; finally establishing the depurating craft of the Lotus leaf.(3)To determine relative peak area of alismol by high-efficiency liquid chromatography (HPLC ) , inspecting the three different extracting method with water refluxing extraction, the method extracted by water and pepted byalcohol and alcohol refiuxing extraction.(4)Establishing the rat model with Hyperlipoidemia,observating each ratgroup in such indicator :blood biochemical indicator, body mass(BM), Livercoefficient and variation in Liver pathological section after using thedepurated Shan-he-jiang-zhi recipe for 21d.(5)All the experiment above is designed by orthogonal design and the result isanalyzed by statistics method.Results: (1)Determine the craft flow-sheet extracting Crataegus total flavonoids from Crataegus: first extracting it using the refiuxing extraction, then depurating using macroporous resin adsorptive process.(2)Determine the craft flow-sheet extracting the Lotus leaf total alkaloids from Lotus leaf: first extracting it using the alcohol refiuxing extraction, then depurating using macroporous resin ion-exchange resin (IER) separation. (3)Initially determine craft flow-sheet extracting the Alismol from Alisma L. orientale Juzep.: using alcohol refiuxing extraction.(4)The blood-serum total cholesterol (TC), triglyceride (TG)and the low-density Hpoprotein (LDL) in the rat of the high-axungia group are obviously higher than those in the blank group, established the experimental blood fat disorded rat model successfully.(5)The blood-serum total cholesterol in the rat of the large dose group and positive control group is depressed obviously; the blood-serum low-density Hpoprotein in the rat of the large dose group ,middle dose group and positive control group is depressed obviously; the blood-serum triglyceride in the rat of the large dose group and positive control group is depressed obviously; the body weight of the rat in every group is lost obviously; the Liver indicatrix is depressed obviously only in the rat of the large dose group, while the high density Hpoprotein (HDL)and the very low density Hpoprotein(VLDL) has not any visible loss.(6)The variation in Liver pathological section presented that: adipose degeneration has happened in the liver cells of the rat in the model group ;liver cells endochylema puffing in the rat of the large dose group ,the middle dose...