The Study Of Kinetics And Mechanism Of Model Complexes Of Hydrolytic Enzyme

Metal ions are involved in many hydrolytic enzymes which can hydrolysis important substances such as protein, phosphate and DNA and play extreme roles in the biochemistry reaction. For the complexity of metalloenzyme's structure, weak stability and the explanation to experiment results with trivial details, model molecules afford wild possibilities to research the vital process.Model enzymes are some kinds of artificial compounds having some enzyme's properties and these compounds are formed by metal ion coordinated with poly dentates ligand. They are designed according to the special relations and coordination of metal-amino acid side chain in the active centre in known enzymes. These compounds with the main active groups and similar special structures of enzymes can imitate enzyme's some critical functions. With simple and easily controlled structure, model compound's spectrum, structure and its biochemistry functions is convenient to study. It is directly to investigate the factors relating to the functions of enzyme by studying the catalyzing behavior of model molecules imitating enzyme's functions. And it is a base of synthesizing high performance model enzyme.Recently the active centre structure and catalytic mechanism of carbonic anhydrase, carboxypeptidase and phosphatase is a hot topic in bioinorganic chemistry.In this paper, bis(dien)zinc(II), bis(dien)copper(II), bis(histidino)zinc(II) , zinc(II) complex of marcocyclic ligand BDBPH are used as model hydrolytic enzymes to study the kinetics and mechanism of these compounds catalytic hydrolysizing p-nitrophenyl acetate. Then the kinetic of bis(dien)zinc(II), bis(histidino)zinc(II), zinc(II) complex of marcocyclic ligand HDTH hydrolysizing p-nitrophenylphate(NA) are measured and the results are discussed. This paper contains seven parts:1. Some related concepts are introduced. The significance and recent developmentof the study of model hydrolytic enzyme's coanpouads are reviewed.2. The principles of the experiment aad measurement method ane concisely.3. The kinetics of the complex of bis(diea)zine(n) catalyzing NA haWjieeB studied and its mechanism is discussed connecting with the species far Zn2+- dien = (i: 2) and its crystal structure.4. The kinetics of the complex of bis(dien)copper(II) catalyzing NA been studied and its mechanism is discassed coaneeting with the species for Cu2+-dien=l :2.5. The kinetics of the complex of bis(histidino)zinc(II) catalyzing NA have been studied and its mechanism is discussed connecting with the species for Zn2+- his = (1 : 2) and its crystal structure.6. The kinetics of zinc (II) complex of marcocyclic ligaad BDBPH cataiysapfNA has been studied and its mechanism is discussed connecting with the distribution for Zn2+- BDBPH - 1 : 1 .7. The complexs of bis(dien)zinc(II), bis(histidino)zinc(II) and zinc(II) complex of marcocyclic ligand HDTH (Zn2+- HDTH =1:2 ) catalyzing p-mtropbenylpfaate have been studied preliminarily.