Study On The Chemical Constituents And Bioactivity Of Ardisia Chinensis Benth

Yao Medicine Ardisia chinensis Benth, which occur in Shaoguan, Guangdong province, have been studied for the first time in this study. The constituents of Ardisia chinensis Benth have been studied by modern scientific technology. Biological activities, such as antibacterial, antivirus and acute liver injury, of aqueous extracts of Ardisia chinensis Benth and other samples that separated from it have also been studied.Seven compounds were separated from Ardisia chinensis Benth. Their structures were established on the basis of IR, 1HNMR, 13CNMR, and MS spectra to be stigmasta-5, 22-dien-3-ol (I), stigmasta-3--D-glucopyranosyl (II), Amyrin (III), lanosta-7, 9(11), 24-trien-3p-ol (IV), Amyrin(V), lanosta-9(11), 24-dien-3p-ol (VI), lanosta-8, 24-dien-3p-ol (VII). All these compounds were first separated from Ardisia chinensis Benth, and stigmasta-3-p-D-glucopyranosyl (II), lanosta-7, 9(11), 24-trien--3p-ol (IV), lanosta-9(11), 24-dien-3p-ol (VI), lanosta-8, 24-dien-3p-ol (VII) were first separated from Ardisia Sw.The volatile constituents of the essential oil of Ardisia chinensis Benth extracted by steam distillation have been studied by gas chromatography mass (GC-MS). A total of 50 compounds have been identified representing the 90.6% of the oil. The major constituents were found to be alkenes, ketones, alcohols, esters and acids.The antibacterial activities of different menstruum extracts of Ardisia chinensis Benth samples against 6 strains of bacteria such as Escherichia coli were investigated. Minimal inhibitory concentration (MIC) of 5 different menstruum extracts of Ardisia chinensis Benth and 7 samples that separated from Ardisia chinensis Benth to various bacteria was determined by the way of microdilution broth method in 96 wells plates. The results evaluates that the distilled water extracts shows a clear inhibitive effect to Staphylococcus epidermidids (MIC=0.313mg/ml), and the ethyl acetate extracts, the n-butanol extracts and two samples that separated from n-butanol extracts of Ardisiachinensis Benth shows some inhibitive effects to Staphylococcus epidermidids (MIC =0.625).The protective effects of the aqueous extracts of Ardisia chinensis Benth on mice acute liver injury induced by carbon tetrachloride (CCU) have been investigated. The results indicates that compared with those of the untreated group, the aqueous extracts of Ardisia chinensis Benth can significantly decreased the enzyme activities of alamine aminotransferase (ALT) and aspartate aminotransferase (AST) in liver injury in mice caused by carbon tetrachloride(CCl4), as the same effect with the positive control drugs. These enucleate that the aqueous extracts of Ardisia chinensis Benth shows a good protective effect on mice acute liver injury induced by carbon tetrachloride.The anti-HBV viral study of the aqueous extracts of Ardisia chinensis Benth was carried out in vitro by primary duck hepatocyte (PDH) culture system. The primary duck hepatocytes were obtained from DHBV-positive duck through collagenase perfusion, and the DHBV DNA was detected by dot-blot hybridization assay. The result evaluates that the aqueous extracts of Ardisia chinensis Benth shows a obvious effects on duck hepatitis B virus, IC50 is 273.5ng/ml.The above biological activitiy research substantiate that Ardisia chinensis Benth can work for bacterial, HBV virus and acute liver injur, and thus laid the foundations for studying the activity mechanism and relationship between the structure and the activity.