Analysis Of Resulted Cancer Matter Arecoline And Safrole In Edible Betelnut

Betelnut is a kind of staple Chinese traditional medicine, classified medicine andedible betelnut. Edible betelnut, cigarette and chutty have been considered mostpopular mouth hobby staple in many countries of world, and it's elling amount ismore and more large. Betelnut have many officinal values, but often chewing resultsbuccal cancer and leads other diseases according to each kind study. Internationalcancer study center of world sanitation organization affirmed betelnut as stair resultedcancer matter on August 7,2003. The dissertation mainly study and analyze resultedcancer matter arecoline of six betelnut samples, and analyze possibly existing safrolein edible betelnut of native land two main provinces.Firstly, considers several main influencing factors extraction time, extractiontemperature and extraction solvent capacity, then optimize them. Get efficiencyextraction condition, extract time 4h, extract temperature 20oC and aether capacity80mL for 4g nature betelnut or 60mL for 4g betel nut. Then select reasonable GCcondition, and get GC/MS condition of good separate effect. The condition asfollows:The material and size of capillary: fused silica oxygenation coated capillary(30m×0.32mm×0.25μm).Oven initial temperature 0oC,keeping 2min, and promote temperature to 210oC as therate of 10oC/min.MS transfer line temperature 280oC, Ion sourse 280oC.Carrier: high pure helium, carrier flow: 1.0mL/min.Ionization mode: positive ionization, IE70eV; Multiplier voltage: 1246V.Scan range: 50-600m/z; Scan rate: 0.63/s.SSL mode: auto-sampler, SSL temperature: 250oC, splitless time: 0.80min, Contantflow.Analyze six betelnut compouds under above condition, and get their total ionscurrent. Then parse the chromatogram figure at retention time about 10.75min of their itotal ions current. Combining computer searches system and mass spectrum parsingtheory, comfirm quality of arecoline through the peak that shows the compound.After producing arecoline with thin chromatoraphy, and separating and purifyingto get to ure degree 91.84% arecoline. The method RSD is 1.16%. Recovery ofarecoline is between 99.6% and 103.3%. Taking on good linear relative for solutionand peak area within quantificational range. Checking limit is 9 ng/mL anddetermining limit is 22 ng/mL. And arecoline stability is good. Determine sixarecoline contents of six betelnuts through selective ions monitor of internal standardmethod. Compare the results as capacity method. And get GC/MS preceding deal ismore simple. The result also shows the results cancer matter arecoline contents ofbetelnuts is very high (>3%). The arecoline contents of Hunan and Hainan ediblebetelnut are 0.62% and 0.55%.To seperate and extract possibly existing safrole in betelnut in simultaneousdistillation extraction. Under the 90oC and 60-90min of SDE, safrole have goodthermal stability and high extraction recovery. Select 90min as experimental time.Extraction solution is analyzed under experimental GC/MS condition. Sparsechromatogram figures to educe the conclusion that afrole doesn't exist in Hunan andHainan province edible betelnuts under detectable limit 18ng/mL.