Separation And Analysis Of Polysaccharide Extracted From Some Traditional Chinese Herbs

Microwave-Assisted Extraction (MAE) as a novel technique is applied in this dissertation to extract the water-soluble pachyman from poria cocos, the Polysaccharide from Bupleurum chinense and mulberry leaf. MAE is a process of using microwave energy to heat solvents in contact with a sample in order to partition analytes from the sample matrix into the solvent. Uniform design and Orthogonal array design is used to optimize the MAE condition. Quantitative determination of Polysaccharide is undergone by phenol-sulfuric acid method and anthratone-acid method. Additionally, Ultrasonically Assisted Extraction (UAE) and Soxhlet extraction are studied as comparative extract techniques. Statistic analysis and intuitionistic analysis to the experimental data shows that the ability to rapidly heat the sample solvent mixture is inherent to MAE and the main advantage of this technique. The main contents and some conclusions are provided as follows:1. Extraction of the water-soluble pachyman from poria cocos using MAE. Uniform design is applied to find the optimal conditions. The extraction rate was 2.792% under the optimal condition(Extraction time:18min;power of macrowave:42%×800;ratio of solid to liquid:1:50), which is remarkably higher than that of traditional method(which is 1.353% only) and that of UAE(which is 1.706%). Compared with the method added enzyme in reference(extraction time: 100min,temperature:100℃,extraction rate:2.4%), the extraction rate of MAE process is comparable and the time it takes is also greatly shortened. 2. Extraction of Polysaccharide from Bupleurum chinense using MAE, UAE and Soxhlet extraction, in which Uniform design is applied to find the optimal conditions. The extraction rate was 28.46mg/g under the optimal condition in MAE process. The results indicated that the extraction rate of MAE under the optimal conditions was remarkably higher than traditional or ultrasonic-assisted extraction. The extraction time of MAE was greatly shortened as well.3. Extraction of Polysaccharide from mulberry leaf by MAE, UAE and traditional extraction method. Uniform design and orthogonal array design are used to find the optimal conditions. UAE technique is comparable with MAE process in extraction rate and extraction time. But the advantage of MAE is obviously when compared with traditional method. However, the advantages relative to other extraction techniques are not so obvious as the extraction of polysaccharide from poria cocos and Bupleurum chinense.Polysaccharide extracted from poria cocos, Bupleurum chinense and mulberry leaf is furthered separated and analyzed by Capillary electrophoresis after sample preparation and derivation. The results show that the MAE process is rational and confirmed to be efficient, which should be considered to be an excellent technique of sample preparation, extraction of micro-component from Chinese herb and extraction of pesticide residue from soil matrix.