Polysaccharides Of Waste Material Extraction And Application

This paper studies the hot water extraction ofpolysaccharides optimum conditions Waste Material major responsesurface method, and try to microwave-assisted extraction and applicationof ultrasonic assisted extraction with hot water extraction, and comparesthe two improved methods effect. Optimal conditions for hot waterextraction which is accomplished by optimizing the response surfacemethod to optimize the results of microwave-assisted extraction andultrasonic assisted extraction is completed by a single factor variablemethod, the polysaccharide concentration detection polysaccharideextract of adoption phenol-sulfuric acid method. Finally, whether theextract can promote the growth of plant seedlings were tested, using acucumber, tomato, pepper seedlings three crops were grown experiment.The specific contents and results are as follows:1. Hot water extraction optimum extraction conditions optimized usingresponse surface optimization method of thinking. Three factors werefirst single factor experiments, and then it’s on the basis of theexperimental design and three levels of three factors, the selectedtemperature, extraction time and the ratio of water. After analysis of thecorresponding surface Design-Expert software, the optimum extractionconditions were as follows: temperature95℃, extraction time4.14h,water feed ratio14.96:1, polysaccharide extraction rate of19.85%..Taking into account the practical application of the simplicity, theconditions are corrected to95℃,4h,15:1. Waste Materialpolysaccharide content in this conditions is1.35mg/ml.2. Using microwave-assisted heating methods on traditional hot waterextraction has been improved. Based on the original best conditions onthe single factor variable experiment, the only variable is the microwaveirradiation time. The result is: the ability to significantly improve theextraction efficiency WMLE polysaccharides microwave irradiation50min. Polysaccharide improved extraction rate of23.8%. Examine theimpact of ultrasound on WMLE polysaccharide extraction efficiency is also used unilabiate method, the only variable is the ultrasonic power.The result is: Based on the original best conditions on the use ofultrasonic power200w, Waste Material polysaccharide extracted underthese conditions was22.3%. Microwave-assisted extraction andultrasonic assisted extraction were improved compared with conventionalextraction methods. Comparison of two methods to improve theextraction rate and production costs, the effect of microwave-assistedextraction on superior ultrasonic assisted extraction. In the process oflarge-scale industrial production of microwave assisted extractionadvantage.3. By the three crop seedlings (cucumber, tomato, peppers) administeredWMLE polysaccharide extract and observed their growth targets,conclusion: applying WMLE polysaccharide extract seedlings comparedwith the control group, more developed root system, higher plants, stemsthicker, higher seedling index. This shows that WMLE polysaccharideson cucumber seedlings, tomato seedlings, pepper seedlings have grownpromote.