Resonance Scattering Spectral Studies On Gold, Gold-Cationic Surfactants And Protein Etc Nanoparticles Systems And Their Analytical Applications

Part Ⅰ Introduction Some basic knowledge of historical investigation of light scattering and resonance scattering wereintroduced. This paper reviews the progress of the resonance scattering technique studies and theiranalytic application in recently.Part Ⅱ Relationship Between Resonance Scattering and Resonance Absorbance of Gold Nanoparticles When the concentration of gold nanparticles in size of 10nm is less than 35.4μg/ml Au, there area max absorption peak at 525nm and a resonance scattering peak at 525 nm. It exhibits a maxabsorption peak at 530nm and a resonance scattering peak at 530 nm for 0－17.7μg/ml goldnanoparticles in size of 50 nm. When the concentration is higher, there are red-shift of resonancescattering peak, and resonance scattering quenching. According to the interaction between the excitedphoton and the interface electron of nanoparticles, and repeat scattering among gold nanoparticles, thereasons of the relationship between resonance scattering and resonance absorbance, the red-shift andthe quenching of resonance scattering peaks have been interpreted.Part Ⅲ The change color effect and spectral properties of the system of gold nanoparticles-cationicic surfactants The change color effect of gold nanoparticles solution was studied by using resonance scatteringspectra, absorption spectra and scan electron microscopy. The red Au nanoparticle solution, withdiameter of 10nm, exhibits a resonance absorption peak and a resonance scattering peak at 525 nm.After adding some cationic surfactants, the color of the solution change to blue (namely, it exist changecolor effect) with two resonance absorption peaks at 550nm and 680nm, respectively, and a greatlyenhanced resonance scattering peak at 525nm. The results show that the change color effect of Aunanoparticles solution is associated with the hydrophobicity of cationic surfactants, the increasingdiameter of Au nanoparticles, and change of resonance absorption peak and resonance scattering. 3广西师范大学硕士论文 AbstractMoreover, the critical coagulation concentrations of some electrolytes were measured by resonancescattering spectral technique.Part Ⅳ Fluorescence and Resonance Scattering Spectra Behavior of HSA- Acetone (Ethanol) systems The fluorescence and resonance scattering spectra of pH 7.4 Tris-HCl- acetone (or ethanol)-HSAsystems were studied by double cell procedure. The results demonstrate that acetone has a fluorescencequenching effect on the HSA system at 325nm results from the molecular absorption of acetone.Ethanol has fluorescence enhancement effect on the HSA system at 325nm. And the reason is ethanoldestroyed the structure of HSA, formatted hydrogen bond with HSA and exposed the Trp group to theinterface of HSA. In pH4.8NaAC-HAC buffer solution, when the concentration of acetone is more then30% or the concentration of ethanol is more then 40%, HSA molecules could congregate each otherbecause its structure is destroyed. And the congregation of HSA rapidly enhances the resonancescattering intensity of systems at 470nm.Part Ⅴ Resonance Scattering Spectral Studies on CAS－Al(Ⅲ)－PEG4000 system and its analytical application In the buffer solution of pH5.0 NaAc-HAC, with the non-ion surfactant Polyethylene glycol 4000(PEG4000), the formation of Chrome Azcerol S (CAS)- Al(Ⅲ) complex particles results in a significantenhancement of resonance scattering of the system with two strong resonance scattering peaks at400nm and 530nm. And the enhanced resonance scattering intensity is linearly proportional to theAl(Ⅲ) concentration in the range of 0.084-0.84μg/mL. The Optimum conductions of the reaction andthe tolerance of coexistent su...