Studies On Spectral Properties Of Silver, Cationic Surfactant And Protein Nanoparticle Systems And Their Analytical Application

Part. 1 Spectral Properties of Nanoparticle Systems and Their Analytical Application Introduce the progress of the absorption, fluorescence, resonance scattering and Raman scatteringspectra and their analytic application of nanoparticle systems in the last years.Part. 2 Studies on Spectral Properties of Silver Nanoparticle Yellow silver nanoparticles with their diameter of 20nm were prepared by microwave synthesis withtri-sodium citrate and silver nitrate solution reacted. Study found that there is interface fluorescence effect,interface fluorescence quenching, resonance scattering quenching and hypochromic effect first in the world.Yellow silver nanoparticles have one board fluorescence peak from 300nm to 600nm,the strongest at 465nmand another stronger at 350nm for the silver nanoparticles. The resonance scattering intensity at 465nm,fluorescence intensity at 465nm and absorbance at 455nm were found linear to the concentration in the rangefrom 0 to 3.5×10-4 mol/L Ag, with regression equation for A455nm=1.23×104c+0.01,F465nm=28.71×104c+3.50and I465nm=48.13×104c+3.69 and regression coefficient for 0.9957,0.9954 and 0.9976.According to the abovelinear relation, the new method for measured silver nanoparticle concentration was established. When theconcentration of silver nanoparticles was over 3.5×10-4c mol/L, the resonance scattering peak andfluorescence peak of 465nm take place red shift and self-quenching, but absorption peak place does notchange and the absorption intensity enhances. When diluted the self-quenching yellow silver nanoparticles inthe water, the resonance scattering and fluorescence peaks shift to short wavelength and their intensity firstenhances then decreases. Further study found that the silver nanoparticles do not only have the same moleculefluorescence quenching, but also red shift self-quenching. At the same time, the silver nanoparticles in liquidalso have ultraviolet, visible and IR fluorescence, however it does not have obviously excited characteristicpeak or fluorescence bleaching. The paper reports the spectral properties of silver nanoparticles, and offersthe principle of interface fluorescence and resonance scattering electron energy band, which further states theeffect of silver nanoparticles in liquid phase.Part. 3 Effects of Cationic Surfactants on Spectral Properties of the Silver Nanoparticle System Brilliant yellow silver nanoparticles, with their diameter of 12nm, by means of silver nitrate and NaBH4solution reacted, exhibits a resonance absorption peak at 400nm, a fluorescence peak at 460nm and aresonance scattering peak at 460nm. After adding some cationic surfactants, the color of the solution takesplace to change and color becomes light (namely, it exists change color effect and hypochromic effect). Boththe intensity of resonance absorption band from 460nm to 600nm and resonance scattering peak at 460nm 3广西师范大学硕士论文 英文摘要fluorescence peak at 460nm and Raman scattering at 550nm enhance. When adding concentration of CS overthe above, the change color effect was displayed again and color becomes deep (namely, it exists change coloreffect and hyperchromic effect) and the intensity of resonance scattering peak at 460nm, fluorescence peak at460nm and Raman scattering at 550nm becomes weak. And the resonance absorption band from 460nm to600nm becomes weak, too. The change color effect, enhanced and quenching fluorescence effect were studiedby employing resonance scattering spectra, absorption spectra, fluorescence spectra and transmission electronmicroscopy. The results showed the enhanced,quenching fluorescence effect and change color effect of silvernanoparticle solution have been associated with the hydrophobicity of cationic surfactants. And increaseddiameter of silver nanoparticle and change of resonance absorption peak and resonance scattering result in thechange color...