Study On The Novel Methods For The Determination Of Several Doping Agents By Molecular Spectroscopy

Doping agent is the total name of the forbidden substances in sport field in the world. The intake of doping agents will not only make the sport spirit lose justice, competition and encouragement, but also greatly threaten the health of athletes. Therefore, the studies of establishing sensitive and selective analytical methods to determine doping agents are of great importance. The optical properties of doping agents have been studied in this thesis, based on which, novel methods for their analysis and determination by fluorescence spectrum and Rayleigh light scattering spectrum were proposed. The main work are summarized as following:1. Based on the native fluorescence of propranolol, a simple and sensitive method for its determination was proposed. The effects of solvent, surfactant and acidity on the fluorescence intensity of propranolol were studied. Under the optimized conditions, the fluorescence intensity was proportional to the concentration of propranolol in the range 1.0×10-8~ 3.0xl0-6mol1-1. The limit detection was 1.5×10-9 mo11-1. The proposed method has been successfully applied to the determination of propranolol in tablet and urine.2. The interaction between propranolol and gold nanoparticle was studied by Rayleigh light scattering spectrum, ultraviolet-visible spectrum and transmission electron microscopy. The addition of propranolol to the solution of gold nanoparticle could induce its aggregation and the RLS intensity would increase quickly, based on which, a novel approach for the determination of propranolol by RLS spectrum was proposed. The effects of different buffer solution, pH, concentration of gold nanoparticle and reaction time on the intensity of RLS were discussed. Prorpanolol could be qualitatively determined in urine with the combination of solid phase microextraction.3. Through the interaction of eight doping agents with Triton X-100, it was found that testosterone, methyltestosterone, spironolactone and benzthiazide could quench the fluorescence of Triton X-100. The fluorescence intensity of Triton X-100 decreased proportionally to the concentration of them, based on which, a novel, simple approach for the determination of the four doping agents was proposed. The studies of absorbance spectra indicated that the fluorescence quenching belonged to dynamic quenching type; the constants of quenching were also calculated. The proposed method has been applied to their determination in the synthetic samples withsatisfactory results.4. Adding bromide to Pb solution of N,N'-dimethylformamide (DMF), the highly emissive cluster Pb4Br could be formed and the fluorescence intensity of the formed cluster was proportional to the concentration of Pb, based on which, a novel, simple approach that used the emission from itself as the sensor for determination of Pb was proposed. Under the optimum conditions, the linear range and detection limit were mol and 7.6xlO mol, respectively. The relative standard deviation (R.S.D.) of determining 11 samples containing 1.0 10 mol Pb was 1.6%. The proposed method has been successfully applied to the determination of lead in the synthetic samples. The mechanisms of the formation and luminescence of Pb4Br were also studied.