Effects Of Heavy Metal Ions On Toxicity Of Litopenaeus Vannamei

This paper studied effects of heavy metal ion on toxicity of Litopenaeus vannamei and discussed mainly the following these:(1)Effects of heavy metal ions on Metallothionein concentration in hepatopancreas of Litopenaeus vannamei. (2)Effects of heavy metal ions on SOD activities in tissues of Litopenaeus vannamei.(3)Effects of heavy metal ions on transaminases activities in tissues of Litopenaeus vannamei. 〦ffects of heavy metal ions on Na+-K+-ATPase activity in gills of Litopenaeus vannamei.1. Effects of heavy metal ions on Metallothionein concentration in hepatopancreas of Litopenaeus vannamei.The results were as follows: The MT concentration in hepatopancreas of Litopenaeus vannamei changed significantly with the increasing of metal ions concentration and prolongation of exposure time(p<0. 05) , whereas that in the control groups had no marked changes (p>0. 05). The MT concentration inducted by Cu2+ (0.1 0.2 0.5 1mg/L) Zn2+ (1 2 5 mg/L) and Cd2+ (0.05 0.1mg/L) increased in 96 h with time and changed stably after 48 h. In addition, the MT concentration inducted by Zn2+ (10 mg/L) and Cd2+ (0.25 0.5mg/L) was up to max at 48 h, began to decrease with time passing by, and still higher than that in the control groups. The inductive effects of three kinds of heavy metal ions on Metallothionein concentration were Cd2+>Zn2+>Cu2+.2. Effects of heavy metal ions on SOD activities in tissues of Litopenaeus vannamei.The results indicated SOD activities in three tissues (hepatopancreasgills blood)of Litopenaeus vannamei treated with different metal ions changed significantly with time (p<0. 05). The SOD activities in three tissues in Cu2+-treated groups changed like a wave as time passing by, and those in hepatopancreas were inhibited obviously in Zn2+(10mg/L)-treated groups as well as those of hepatopancreas and gills in Cd2+(0.5mg/L)-treated groups, but no significant changes of SOD activites in gills were found in 0. 25mg/L Cd2-treated groups. Those in three tissues increased at first and then decreased as time passing by in groups tread by other concentration of Zn2+ and Cd2+. Wherereas the SOD activities of control groups didn' t changed significantly (p>0. 05) during experiment times. In addition, the effects of three kinds of metal ions on SOD activities in three kinds of tissues presented obviously dose-time response relationship. SOD activities of three tissues were hepatopancreas> gills > blood and the harm of tree heavy metal ions to Litopenaeus vannamei was Cd2+>Cu2+>Zn2+.3. Effects of heavy metalions on transaminases activities in tissues of Litopenaeus vannamei.The results were as follows: Transaminases (GOT GPT) activities of different experimental groups in three tissues(hepatopancreas gills. blood) changed significantly as time passing by (P<0.05) .And the activities of GOP and GPT decreased obviously in gills and hepatopancreas, but increased obviously in the blood while no marked changes were found in the control groups (p>0.05). In addition, effects of three metal ions in the transaminases activities positively correlated with the concentration of ions and the changing trends were identical on the whole. The inhibition rates of Cu2+ on the two transaminases activities in gills and hepatopancreas were GPT > GOT. The inhibition rates of Zn2+ in hepatopancreas were GPTGOT in the low-dose( 1? mg/L) groups but GPTGOT. However, the activation rates of three meal ions on activities of GPT and GOT in the blood were GPTgills>blood. 4. Effects of heavy metal ions on Na'HC-ATPase activities in gills of Litopenaeus vannamei.The results show that effects of three kinds of heavy metal ions on Na+-K+-ATPase activities in gills were significant (p<0.05) , while the control group was not significant (p>0.05) . The Na...