Studies Of Electrochemical Behavior Of Aciclovir And The Interaction Between Copper (Ⅱ)-Aciclovir Complex And DNA

Adsorptive voltammetry is one of the most important electrochemical analytical methods since it was developed in 1980s. This method that can give high sensitivi ty and satisfactory selectivity is simple, fast and accurate. There is a very important theoretical significance and value of application in studying relative drugs, which connect with human health. Adsorptive voltammetry has been used to study the voltammetric property of acyclovir at glass carbon electrode, which is a kind of anti-virus drug. The determination of the concentration of aciclovir tablets has been finished by this method and the results is satisfactory.Based on the interaction between small molecules and DNA, these research which direct toward the design of specific reagents provide routes toward rational drug design as well as a means of developing sensitive electrochemical probes in relation to DNA. The binding of copper(II)-aciclovir complex with calf thymus DNA has been investigated by voltammetry, UV-Vis spectra and viscosity measurements.The dissertation consists of four chapters. Chapter l:ForwordThe foreword is a comprehensive review on the application andtheoretical research of adsorptive voltammetry in the area of medicalanalysis and the interaction between DNA and small molecules.Chapter 2: Study and determination of aciclovir by adsorptive voltammetryAciclovir is oxidized at a glass carbon electrode by adsorptive voltarametry in BR buffer(pH=3. 0). The peak potential is at about 1. 25V (VS. Ag/AgCl) . The linear range is 5 X 1(T~1. 5X 10~4mol/L, and the detection limit is .2.0 X 106mol/L. Aciclovir tablets have been determined with good results by this method. The voltammetric behavior and reaction mechanism have been studied. It shows that it' s an unreversible process with one electron and one proton transfered atglass carbon electrode.Chapter 3: Study and determination of aciclovir at activated electrode by adsorptive voltammetryAfter activating the glass carbon electrode in Imol/LNaOH for 500 second by cyclic voltammetry, aciclovir has been studied by adsorptive voltammetry at activated electrode. The linear range is 5X10 ~1X 10~5mol/L. The detection limit is 1. 7X 10~'mol/L, it is one seventeen to that at bare electrode. The determination of aciclovir of lowerconcentration has been done and the results is satisfactory.Chapter 4: Study of the interaction between copper(II)-aciclovir complex and DNAIt has been obtained that the complex ratio of copper to aciclovir is 1:2, and the complex stability constant is 2. 34X109 mol~2L2 at the first of this chapter. The interaction of copper(II)-aciclovir complex with calf thymus DNA has been investigated by voltammetry. It has been found that adding DNA to the solution of complex cause decreasing ofpeak currents. The binding mode has also been studied with the assistanceof UV-Vis spectra and viscosity measurements. It shows that complex binds with DNA probably by partial intercalation.