Resonance Light Scattering Of The Determination Of Deoxyribonucleic Acid Triphenlmethane Dyes System And Its Analytical Application

The Resonance light-scattering (RLS) spectra characteristics of interaction of Triphenlmethane Dyes System and deoxyribonucleic acid (DNA) have been studied, based on a lot of documents and the conditions of the laboratory. Four new determination methods of DNA have been established according to the variation of the intensity of RLS which is in direct ratio to the concentration of the DNA in this paper. These methods are characterized by quickness and sensitivity, and are applied to quantitative analysis of fish sperm DNA (fsDNA) and culf thymus DNA (ctDNA). The results are satisfied.There are four chapters in this paper.In the first chapter: The RLS spectra characteristics of interaction of Brilliant Green (BG) and DNA have been studied with RLS method in the medium with the value of pH 6.35. The effects of interference substances and optimization of procedure have been investigated and a new method for determination of the DNA has been established . Based on RLS method, the linear relationships have been studied between the enhanced intensity of RLS at 332nm and concentration of deoxyribonucleic acid in the BG solution, good correlation coefficient 0.9918 have been obtained, and the detection limits are 0.13-3.50 mg/L. The approach has good operation, high sensitivity and accuracy. The method has been applied to determination of fish sperm DNA with satisfactory results.In the second chapter: A new method for the determination of the DNA have been established on the basis of RLS technique. Characteristics of interaction of Victory Blue-B(VB-B) and DNA have been studied in the medium with the value of pH 9.81 by RLS technique. The effects of interference substances and the condition of the reaction on the system have been investigated and the optimalcondition is presented. The linear relationship have been studied between the enhanced intensity of RLS at 355nm and concentration of deoxyribonucleic acid in the VB-B solution, good correlation coefficient 0.9978 have been obtained, and the detection limit is 21.5ug/L. The linear range of the DNA's concentration is 0.11-3.00 mg/L. The approach has the characteristics of good operation, high sensitivity and accuracy and wide determination range. It has been applied to determination offish sperm DNA with satisfactory results.In the third chapter: The RLS spectra characteristics of interaction of Methyl Violet 6B(MV-6B) and DNA have been studied with the RLS method in the medium with the value of pH 2.23. The linear range of the fsDNA is 0-1.00 mg/L and the ctDNA is 0-1.50 mg/L with the correlation coefficient of 0.9993 and 0.9997. The detection limit is 15.3 ug/L and 12.2 ug/L. The method has been applied to determination of DNA in mixed samples with satisfactory results.In the fourth chapter: Resonance Light-scattering (RLS) characteristics of interaction of New Magenta(NM) and Deoxyribonucleic Acid (DNA) have been studied in the PH 0.85 medium. The effects of interference substances and optimization of procedure have been investigated and a new method has been established for the determination of the DNA (fsDNA, ctDNA) at nanogram level, based on RLS measurement. The linear range of the fsDNA is 0.02-6.00 mg/L, and the ctDNA is 0-7.00mg/L with the correlation coefficient of 0.9984 and 0.9987. The detection limit is 18.3 ug/L and 21.8 ug/L. The method has been applied to determination of DNA in mixed samples with satisfactory results. Meanwhile we have also discussed this reaction mechanism. The formation constant between New Magenta and DNA have been obtained by Ultraviolet-Visible spectra.