| Nucleic acid is the essential genetic material, which plays an important role in the life science. The research of nucleic acid is very important in biology, biochemistry and medicine field.In the chemistry research field of nucleic acid, it has been very active that the small molecules is used as anticancer drugs, the dye of nucleic acid and construction probe. Chemistry with its advanced equipments and sensitive detection means provided the solid foundation for the research of the nucleic acid.Resonance light scattering (RLS) is a new technology developed in recent years. For its remarkable characteristics of high sensitivity, high selectivity and simple operation, this method brings to more attention and interesting and has been applied widely to the determination of biological macromolecules such as nucleic acids and proteins. Resonance light scattering technique simplified the equipments of the light scattering analyses, is easy to operation and suitable to the assay of large quantity samples. Resonance light scattering technique has exhibited the extensive and practical worth and wide appliance foreground in chemical analysis.In the thesis, several new determination methods of DNA are developed by means of resonance light scattering technique, and the interaction mechanism between DNA and anticancer drugs is also investigated. This thesis is composed of three chapters:The first chapter: Studies on resonance light scattering spectra of DNA in organic acid medium and it's analytical application.The different enhancement of resonance light scattering by calf thymus DNA is observed in formic acid, acetic acid, butyric acid, trichloroacetic acid, and trifluoroacetic acid. However, in trichloroacetic acid medium, the resonance light Scattering intensity of calf thymus DNA has a maximum value and best stability. The concentration of calf thymus DNA in the range of 0.05-50 ng/mL and Fish sperm DNA in the range of 0.05-40 μg/mL is well proportional to the enhanced resonance light scattering intensity the 0.2 mol/L trichloroacetic acid solution. The detection limit is 12.24 ng/mL for calf thymus DNA and 17.94 ng/mL for fish sperm DNA, respectively. Nucleotides at 4μg/mL, proteinsat 0.1 μg/mL, and most of metal ions at 1.0×10-5mol/L do not interfere with the determination of DNAs. Based on this, a new method for determination of DNA is developed. This method has been applied to the determination of calf thymus DNA and fish sperm DNA in four mixed samples successfully.The second chapter: Study on interaction mechanism of DNA and adriamycin by resonance light scattering spectra and it's analytical application.We find that the resonance light scattering of adriamycin is greatly enhanced by calf thymus DNA in a Britton-Robinson(BR) buffer in the pH range of 2.21-11.98. There are two resonance light scattering peaks at 322nm and 564nm. At 322nm, the enhanced resonance light scattering is well proportional to the concentration of nucleic acids in the range of 0.1-8μg/mL for calf thymus DNA and 0.08~8μg/mL for fish sperm DNA, respectively. The detection limit is 36.8 ng/mL for calf thymus DNA and 40.1 ng/mL for fish sperm DNA, respectively. The proteins at 10 #g/mL, nucleotides at 10μg/mLdo not interfere with the determination of DNAs, most of metal ions can be tolerant at very high concentration (> 10-5 mol/L). A new method for determination of DNA was established. It is demonstrated that the determination method has the higher selectivity, due to the high specificity of interaction between DNA and adriamycin.The third chapter: Study on the interaction of DNA and polyamine by using resonance light scattering spectra and it's analytical application.The interaction of DNA and three kinds of polyamine is studied by means of resonance light scattering spectra. It is found that the resonance light scattering of spermine can be greatly enhanced by calf thymus DNA in a Britton-Robinson buffer of pH range of 2.21-9.15. There is a resonance light scattering peak at 343 nm. The concentration of calf thymus DN...
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