The Extraction Of Bluegrass DNA And Soybean DNA And Their Interaction With BTMPA

Consulting and reading a great number of references, the interaction of 2-(2-benzothiazolyloxy)-N-methyl-N-phenyl-acetamide (BTMPA) with bluegrass DNA and soybean DNA were studied by UV spectrophotometry, and a novel grass-removal mechanism was proposed.1. The conditions for extraction of bluegrass DNA were optimized by SDS method, The optimal experimental conditions for extraction of bluegrass DNA were as following: 4.0 mL of cell extraction solution, 0.4 mL of absolute alcohol, 40 min of heat time at 65℃C, 1.0 mL saturation NaOAc solution, the rate between the phenol/chloroform/isoamyl alcohol, chloroform/isoamyl alcohol and isopropanol and the test solution being 0.6, 0.9 and 0.7, respectively. Under this optimal conditions , Productivity of bluegrass DNA is 381 μglg and the value of A260/ A280 is between 1.76¹.82, DNA is very pure.2. The optimum conditions for soybean DNA were as following: 5.0 mL of cell extraction solution, 0.5 mL of absolute alcohol, 30 min of heat time at 65℃, 1.0 mL saturation KC1 solution, the rate between the phenol/chloroform/isoamyl alcohol, chloroform/isoamyl alcohol and isopropanol and the test solution being 0.5, 0.6 and 1.0, respectively. At this optimal conditions , Productivity of soybean DNA is approximately 900 μg/g and the value of A260/ A280 is between 1.75 1.81.3. UV spectrophotometry the interaction of BTMPA with bluegrass DNA or soybean DNA was studied, we observed that when BTMPA was used as herbicide for xerophils, it can intercalate double strands DNA of bluegrass and soybean and destroy the partnership of bases, making the double strands of DNA unwound and inhibiting the replication of DNA, so the weeds were killed. Using BTMPA as pesticide the optimum range of BTMPA concentration is 3.08×l0^-5mol/L 6.16×10^-5mol/L, the optimum range of temperature is 25℃³0℃ and the most proper period is from 1-leaf to 3-leaves.