Mefenacet Paddy Plant Dna Interaction

On the base of consulting a great a mount of references the interaction of 2- (2-benzothiazolyloxy) -N-methyl-N-phenyl-acetamide with paspalum DNA and oryza DNA was studied by UV spectrophotometry and voltammetry. A novel grass-removal mechanism, that the BTMPA can stack on the surface of plant DNA molecules to inhibit the replication of DNA and to eliminate the paspalum, was recommended.1. The conditions for extraction of paspalum DNA was optimized. The optimum conditions were as following: 6.0ml of cell extraction solution,0.6ml of absolute alcohol, 60 min of heat time at 65 C,1.0ml of saturation NaAc solution, the rate between the phenol/chloroform/isoamyl alcohol, chloroform/isoamyl alcohol and isopropanol and the test solution being 0.6,1.0 and 0.6, respectively.2. The optimum conditions for extraction of oryza DNA were as following: 4.0ml of cell extraction solution, 0.4ml of absolute alcohol, 30 min of heat time at 65癈, 1.0ml of saturation NaAc solution, the rate between the phenol/chloroform/isoamyl alcohol, chloroform/isoamyl alcohol and isopropanol and the test solution being 0.5,0.5 and 0.8, respectively.3. The interaction of BTMPA with paspalum DNA and oryza DNA was studied by UV spectrophotometry. The optimum conditions for the elimination of paspalum were as following: the BTMPA concentration range from 1.7 X 10-5-5.1 X 10"5mol/L, the temperature of 20-30 C and the growing period for applying pesticide being from seed to 3-leaves.4. The interaction of BTMPA with paspalum DNA and oryza DNA was also studied by voltammetry. The BTMPA concentration larger than 5.1X 10-5mol/L the BTMPA formed a electrochemical active complex with paspalum DNA, the voltametric peak potential being -1.61V. The BTMPA concentration larger than 6.8 X 10-5mol/L the BTMPA formed a electrochemical active complex with oryza DNA also. So using BTMPA toeliminate the paspalum The optimum conditions were as following: the BTMPA concentration about 5.1 10~5mol/L, the temperature about 25癈 and the growing period being from seed to 3-leaves.